Luminex xMAP® Technology

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New to Multiplexing Using the Luminex Platform? Here’s How Trusted xMAP Technology Brings Your Biomarkers to Life.

Luminex technology, the trusted, widely used platform for biomarker screening and protein analysis, consists of bead-based immunoassays, detection instruments (including compact multiplexing instruments), and software. As a Luminex® partner, we are a preferred distributor of Luminex® instruments, accessories and software. Our ongoing dedication and industry-leading experience with multiplex technology enable you to gain more information faster and reliably.

Advantages of Luminex xMAP® Technology Compared to Other Immunoassay Methods:

  • Speed/High throughput: Simultaneously measure the concentration of a large number of different analytes in a single sample, enabling you to do your work faster, gaining early and comprehensive data so critical to your work.
  • Accuracy: xMAP® technology generates real-time analysis and accurate quantification of antibody-antigen interactions.
  • Reproducibility: High-volume production of xMAP® microspheres allows assay standardization that solid-phased flat arrays cannot provide.
  • Low sample volume: With minimal hands-on time, you can screen more than 40 analytes in a single sample using as little as 25 μL.
  • Magnetic bead-based format: Responds rapidly and efficiently to a magnetic field, enabling better and faster washing techniques, including high-throughput options

How Luminex xMAP® Technology Works

The Luminex® system is the combination of four core technologies:
xMAP® microspheres:
  • xMAP® magnetic bead microspheres are a family of 80 fluorescently dyed 6.45 μm magnetic microspheres that act as both the identifier and the solid surface to build the assay.
  • Traditional agarose microspheres are a family of 500 fluorescently dyed 5.6 μm polystyrene microspheres that act as both the identifier and the solid surface to build the assay.
Bead-Based Immunoassays:
  • MILLIPLEX® MAP multiplex assays consist of analyte-specific capture antibodies conjugated to xMAP® beads, enabling multivariate analysis of complex biological states, including metabolic disease, immunology, neurodegenerative disease, toxicity, cancer and more, using minimal sample volumes. MILLIPLEX® MAP assays are analytically validated for sensitivity, specificity, reproducibility and wide dynamic range.
Luminex® Analyzer:
  • Luminex 200™ and FLEXMAP 3D® systems: flow cytometry-based instruments that integrate key xMAP® detection components, such as lasers, optics, advanced fluidics and high-speed digital signal processors.
  • MAGPIX® system: a CCD-based, compact multiplexing instrument that integrates key xMAP® capture and detection components with the speed and efficiency enabled by magnetic beads.
Software:
  • XPONENT® software package for data acquisition.
  • MILLIPLEX® Analyst 5.1 software package for sophisticated data analysis.
Luminex multiplexing technology consists of xMAP bead-based immunoassays that can be analyzed using two types of instruments: flow cytometry-based analyzers (left schematic) and LED-based analyzer (right schematic). LED-based detection is used in compact multiplexing instruments like the MAGPIX® system.

Luminex multiplexing technology consists of xMAP bead-based immunoassays that can be analyzed using two types of instruments: flow cytometry-based analyzers (left schematic) and LED-based analyzer (right schematic). LED-based detection is used in compact multiplexing instruments like the MAGPIX® system.