DNA Purification

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MagPrep® Silica Affinity Particles

The MagPrep® Silica Particles are designed for rapid isolation of genomic DNA or leukocytes. The beads have a diameter of 100 nm and are provided as a aqueous suspension of 50 mg/ml. The small size of the beads ensures that they remain suspended in solution during the binding process and the high magnetite content (> 95%) allows rapid separation from the aqueous phase using a magnet.

More About MagPrep® Silica Affinity Particles

Polymerase chain reaction (PCR) involves replication of the DNA template by a thermostable DNA polymerase. Downstream manipulations of PCR products, such as restriction digests, ligation, sequencing or hybridization, often require purification of the amplified DNA. Alternatively, PCR products can be directly ligated in plasmids, transformed and propagated in bacteria. Use schematic illustration below to help plan your workflow.

Whether you choose our PCR purification tools and gel extraction kits to obtain DNA fragments with the right purity and concentration for the next step, or whether you opt for one of our easy subcloning kits to preserve your product, you can expect superior recovery, accuracy, and efficiency.

Guide to Cloning Kits

Product Application
Vector
Application
Vector Features
AccepTor™ Vector Kit
(for PCR products from non-proofreading DNA polymerase
pSTBlue-1
  • Archiving
  • Subcloning
  • Sequencing
  • In vitro-transcription
  • SP6/T7 promoters
  • Blue/White screening
  • Amp & Kan selection
  • Dual EcoR1 sites
pETBlue™-1
  • Protein Expression
  • No fusion tags
  • Blue/White screening
  • IT7/lac-driven, tightly controlled, high-level expression in E.coli
Perfectly Blunt® Cloning Kits
(any DNA polymerase product since kit includes end conversion mix for blunt DNA))
pSTBlue-1
  • Archiving
  • Subcloning
  • Sequencing
  • In vitro-transcription
  • SP6/T7 promoters
  • Amp & Kan selection
  • Dual EcoR1 sites
pT7Blue-3
  • T7 promoter
  • Amp & Kan selection
    Dual EcoR1 sites
pT7Blue T7
  • T7 promoter
  • NdeI/BamHI sites flank insert
  • T7 driven In vitro protein synthesis

Merck:/Freestyle/BI-Bioscience/Genomic-Analysis/painted-pellet-dna-purification.jpg
Pellet Paint® pellet (2 mL) under UV and visible illumination.

Pellet Paint® Co – Precipitant (Catalogue No. 69049)

A visible dye-labeled carrier of nucleic acids for rapid, quantitative nucleic acid precipitation

Features:
  • Direct visualization and tracking of alcohol precipitated nucleic acids 
  • Efficient precipitation of DNA or RNA from dilute solutions (2 ng/mL) 
  • Prevents loss of DNA or RNA pellets 
  • Compatible with most procedures including Cy5® sequencers. Recommended for Applied Biosystems automated sequencers 
  • Compatible with more applications
More about Pellet Paint® Co – Precipitant (Catalogue No. 69049)

Pellet Paint® NF Co-Precipitant (Catalogue No. 70748)

Non-fluorescent, visible nucleic acid co-precipitant for automated sequencing applications. 

Features:
  • Non-fluorescent dye-labeled carrier 
  • Efficient and rapid precipitation of BigDye® cycle sequencing products 
  • Efficient removal of dye terminators 
  • Direct visualization and tracking of precipitated material 
  • No effect on sequencing reaction 
  • Compatible with fluorescent detection systems
More about Pellet Paint® NF Co-Precipitant (Catalogue No. 70748)

Spinprep PCR Clean-Up Kit
Column binding capacity: up to 6 µg
PCR sample volume: 100 µL/rxn
Typical recovery: 60-90%
Size range: 100 bp to > 12,000 bp
Time required: < 10 min

SpinPrep™ PCR Clean-Up Kit

Combine simplicity, speed, range and sensitivity when you use this kit to clean up and recover PCR-amplified DNA. The 3-step, 10-minute spin column procedure involves adsorption of DNA to silica membrane activated with binding buffer. After a wash step, DNA is eluted using a low-salt buffer. Effectively remove DNA polymerases, dNTPs, salts and primers from the PCR reaction mix to avoid interference with downstream applications such as cloning, sequencing or labeling. Purify PCR products from 100 bp to > 12 kbp with up to 90% recovery.

More about SpinPrep™ PCR Clean-Up Kit

SpinPrep Gel DNA Kit
Column binding capacity: up to 20 µg
Gel slice mass: 150 mg/rxn
Typical recovery: 50-90%
Size range: 150 bp to > 12,000 bp
Time required: < 30 min

SpinPrep Gel DNA Kit

Quickly and efficiently extract DNA fragments from 150 bp to >12,000 bp from agarose gels without organic extraction or alcohol precipitation. The procedure uses GelMelt™ Solution to dissolve the gel slice, followed by adsorption of the DNA to a silica membrane in a spin column format. After a wash step, the purified DNA is eluted in low-salt buffer.




Gel analysis and quantification of DNA frag-ments isolated with the SpinPrep Gel DNA KitGel analysis and quantification of DNA frag-ments isolated with the SpinPrep Gel DNA Kit

Known amounts (2 μg) of four DNA fragments of the indicated sizes were run in separate lanes on a 1% agarose gel. Each band was excised and the DNA extracted from the gel using the SpinPrep Gel DNA Kit and standard protocol. Recoveries shown as percentages above were determined by absorbance at 260 nm. Samples (250 ng) of each recovered band were analyzed by agarose gel electrophoresis. Lane 1 contained a mixture of the starting DNAs.

SpinPrep Gel DNA Kit (Catalogue No. 70852)

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