MilliporeSigma’s PluriSTEM™ Human ES/iPS Medium is a specially formulated defined media that maintains human pluripotent stem cells in feeder-free and serum-free conditions with less frequent feeding and cell culture time. The proprietary formulation utilizes Activin-A, TGFβ1, and b-FGF to promote stem cell self renewal and potent small molecule combinations to inhibit unwanted spontaneous differentiation along with HSA to aid in overall colony morphology.
PluriSTEM™ Recombinant Vitronectin is a truncated version of human vitronectin protein and is an effective xeno-free alternative to Matrigel for human ES and iPS cell culture using PluriSTEM™ Human ES/iPS Medium, E8, MTESR or NutriStem human ES cell growth media in feeder-free culture conditions.
Dispase-II has proven to be a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro. Reliably passage your human ES/iPS cells with our ready to use Dispase-II Enzymatic solution proven to work alongside PluriSTEM™ Human ES/iPS Medium.