The Simplicon™ RNA Reprogramming Technology utilizes a single, polycistronic, synthetic, self-replicating RNA strand that is sufficient for generating high numbers of human iPSCs with a single transfection step. The single RNA contains the four reprogramming factors, OCT-4, KLF-4, SOX-2 and GLIS1, and allows for efficient reprogramming without viral intermediates or host genome integration.
With Simplicon™ RNA Reprogramming Technology, you can:
Create integration-free iPS cells using synthetic RNA
Reprogram efficiently and easily using a single transfection step
Achieve controlled elimination of reprogramming factors