Flow Cytometry Applications - Immunology
|Tissue-Specific Immune Environments
The immune system, which mediates the body’s response to the introduction of foreign material, is made up of multiple cell types collectively called lymphocytes. Lymphocyte subtypes include B cells (which secrete antibodies), cytotoxic T cells, helper T cells (which secrete cytokines), and natural killer (NK) cells. Characterization of lymphocyte subtypes and cytokine signaling is essential for understanding the complex nature of the immune system.
Guava easycyte™ systems offer rapid interrogation of a variety of common immunologic assays, with up to 8 parameters of detection. Amnis imaging flow cytometers can readily analyze more complex assays, with up to 5-12 parameters, including visual verification of specific cells in suspension and rare events.
Multiparametric phenotypic analysis by flow cytometry allows researchers to study the dynamics of immune signaling in intact cells, and offers many unique capabilities, such as:
|3-Part Differential with antibody confirmation (lymphocytes red CD3, monocytes yellow CD14, granulocytes green CD15). Adult human blood stained with CD3PECY5, CD14-PE, and CD15-FITC lysed with the guava® lysing solution and acquired on the guava easyCyte™ instrument. Data displayed shows clear identification of the 3 major white blood cell populations.|
Guava® AutoCD4/CD4% is an application for enumeration of CD4+ Human T cells in whole blood samples. This application is offered on a dedicated use instrument platform. It provides counts of CD4+ T Lymphocytes in whole blood as well as determination of the number of CD4+ T-cells as a percent of Total Lymphocytes.
Download our data sheet on AutoCD4 HERE.
T-cell mediated cytotoxicity plays a critical role in down regulation of the immune response. Moreover, immunosuppressant compounds often mediate their effects through immunotoxicity. We have developed a broad range of kits for evaluation of immunotoxicology through a variety of mechanisms.
In the example to the right, PBMCs were treated with 80 different cytotoxic compounds for 20 hours. Cells were analyzed with the FlowCellect™ Human T Cell Apoptosis and CD4 T Cell FAS assays followed by microcapillary cytometry. Levels of apoptosis, assessed by Annexin V response, in CD4 and CD8 subsets for multiple compounds are depicted in heat map. The table summarizes compounds that demonstrate significant apoptosis and the % of CD4 and CD8 T cells that underwent apoptosis.
Download our poster on immunotoxicology HERE.
Memory B cells represent approximately 30-60% of the B-cell pool in healthy donors. B-cell subpopulations are reported to be defective in patients suffering from immuno-deficiency disorders, which are correlated with reductions in numbers of circulating CD19+CD27+ memory B cells. For evaluation of Human B cells, we now offer a variety of options.
Download our poster on human B cell function HERE.
GPCR surface identification flow cytometry kits provide high quality, reproducible data in far less time than traditional methods for chemokine receptor quantitation, while avoiding the hazard and expense of radioligand binding assays. Now you can identify and quantify GPCRs on the surface of any cells using a GPCR-specific antibody validated for flow cytometry, and use the included positive and negative controls cells. EMD Millipore offers FlowCellect™ Kits for quantitation of Chemokine Receptor Surface Expression.
Download our poster on quantitation of chemokine receptor expression HERE.