Flow Cytometry Kits for Cell Health

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Featured Cell Health Product
FlowCellect™ MitoDamage Kit
Two-parameter analysis can achieve target-specific detection of phosphorylation, and eliminates false positives in a mixed cell population.
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Analyzed Dual Parameter Data: Dot plots depicting Jurkat cells treated with multiple inducers and stained using MitoDamage Kit. Jurkat cells were treated with 2 µM Staurosporine or 50 µM CCCP and then stained using the MitoDamage Kit. Plots show the percentage of positive cells for 1) Apoptosis (Annexin V binding) and mitochondrial membrane potential change (Red2), 2) Cell death and mitochondrial membrane potential change, and 3) apoptosis and cell death.

ViaCount® Assay Kits

Description Catalogue No.
guava ViaCount® Reagent 100 Tests 4000-0040
guava ViaCount® Reagent 600 Tests 4000-0041
guava ViaCount® Flex Reagent Kit 100 Tests 4500-0110
guava ViaCount® Flex Reagent Kit 500 Tests 4700-0060
guava ViaCount® Cell Dispersal Reagent for Flow Cytometry 100 Tests 4700-0050

Mitochondrial Analysis

A variety of FlowCellect™ Kits can now be employed to assess changes in mitochondrial membrane potential, apoptosis as measured by Annexin V binding, mitochondrial oxidative stress, and cell death.

Features include:

  • Designed for use with flow cytometry systems equipped with 488 nm & 644 nm lasers.
  • Optimized protocols reduce steps and require no compensation before or after acquisition

Mitochondrial Health Kits and Assays

Description
FlowCellect™ MitoPotential Red Kit
FlowCellect™ MitoDamage Kit
FlowCellect™ MitoStress Kit
FlowCellect™ Cytochrome C Kit
FlowCellect™ Oxidative Stress Characterization Kit
guava Mitochondrial Depolarization Assay Kit

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Cell Growth & Tracking

The Guava EasyCyte™ Cell Growth reagent uses a simple, non-radioactive protocol for measurement of cell growth by a combination of carboxyfluorescein diacetate succinimidyl ester (CFSE) and Propidium Iodide (PI). The kit can be used to measure growth out to five generations.

Applications for EasyCyte™ Cell Growth Reagent:

  • Track division in primary cells or cell cultures
  • Screen for compounds that affect cell proliferation
  • Verify health of cell cultures by monitoring cell division

Ordering Information

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Epigenetics and Gene Regulation

Description
Anti-MAD2A-FITC, clone 17D10
Anti-Y14-FITC, clone 4C4
Anti-FXR1-FITC, clone 6BG10
Anti-SMN-FITC, clone 2B1
Anti-CAF1 p150-FITC, clone SS1, 1-3
Anti-TBX21/ T-Bet-FITC
Anti-RPA2 p34 -FITC, clone RPA20 1-46
Anti-RPA1 p70 -FITC, clone RPA9, 1-30
Anti-BMI1-FITC, clone AF27
Anti-RBMS1-FITC, clone 4D11
Anti-c-Jun-FITC, clone 6E4
Anti-RBMS1-FITC, clone 4D11
Anti-BrdU-Alexa 488
Phospho-ATM (Ser1981) - PE
Phospho H3 (Ser10)- Alexa 488
Phospho-SMC1 (Ser957) - Alexa 488
Anti-Cyclin B1 - PE Human

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Autophagy Kits

MilliporeSigma’s FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kits provide a quantitative solution for studying autophagy and measuring potency of autophagy inducers using flow cytometry.

Two host cell lines are offered in either CHO (Chinese Hamster Ovary) or U20S (human osteosarcoma) to assist in your autophagy research. The CHO reporter cell line is ideal for flow cytometry applications, while U20S is suitable for both imaging and flow cytometry.

The following products are now available for use to evaluate autophagy:

Description
FlowCellect™ GFP-LC3 Reporter Autophagy Assay Kit (CHO)
FlowCellect™ RFP-LC3 Reporter Autophagy Assay Kit
FlowCellect™ Autophagy LC3 Antibody-based Assay Kit
FlowCellect™ Autophagy Detection Reagent Pack

Features:

  • Format uses GFP-LC3 stable reporter cell line with monomeric GFP for detecting the rate of autophagy by flow cytometry
  • Precise and quantitative method utilizes selective permeabilization and lysosomal inhibition to remove cytosolic LC3 while retaining lipidated LC3 for detection once it enters the autophagosome.
  • Reusable cell line and reagent pack provides low cost per sample View user guide for Autophagy kits

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Apoptosis

When performed on the guava easyCyte™ system, apoptosis kits can determine the count of a cell population without using external bead sets. Multiparametric evaluation of apoptotic status is of great utility in studying impact of compounds on in drug screening studies. These kits provides a complete means of apoptotic status and its response for inducer treatment conditions or diseases.

Description
Early Apoptosis Kits
FlowCellect™ Annexin Red Kit (Annexin V-CF647 Reagent/7-AAD)
guava Nexin Reagent (Annexin V-PE/7AAD)
guava Nexin Reagent (500 test)
Mid Apoptosis Kits
guava Caspase 8 FAM Kit
guava MultiCaspase SR and Caspase 3/7 FAM Kit
guava MultiCaspase SR Kit
guava MultiCaspase FAM Kit
guava Caspase 3/7 FAM Kit
Late Aptoptosis Kits
guava TUNEL Reagent Kit (Anti-BrdU-TRITC/ TdT Enzyme/ Br-dUTP)
Apoptosis Signaling Kit
FlowCellect™ Bcl-2 Activation Dual Detection Kit (Anti-Bcl-2-Alexa Fluor 488/Anti-pBcl-2 (Ser70)-PE)

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Cell Cycle

Cell cycle phase distributions can be used to assess cell health, proliferation, as well as the potential mechanism of antineoplastic agents. For example, measuring the population of S phase cells can reflect the amount of newly synthesized DNA. Also, distinguishing cells in G2 from M phase cells can help identify cells undergoing mitosis.

Guava and FlowCellect™ Kits for Cell Cycle provide many advantages for evaluation of cell cycle.

  • Combines directly conjugated antibodies and/or fluorescent dyes and protein reporters
  • The kits also contain complete buffer sets, protocols and pre-defined gate settings so they require no compensation.
  • They are fully optimized for "plug-and-play" cellular analysis on Guava easyCyte™ and other flow cytometry instruments.
Description
FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis
FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis
guava Cell Cycle Reagent