Amnis® Instrument Comparison

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Explore our Imaging Flow Cytometers

Amnis® imaging flow cytometers are available on two platforms: the FlowSight® Imaging Flow Cytometer, and the ImageStream®X Mark II Imaging Flow Cytometer. Our cellular specialists are here to help determine the best instrument for your research and lab needs.


Product SpecificationsFlowSight®ImageStream®X Mark II
Description Compact, high-sensitivity, multi-color flow cytometer. Produces multiple images per cell for qualitative and quantitative, statistically robust image-based data.
Learn more about FlowSight®
High-resolution microscope for suspended cells. produces detailed brightfield, darkfield, and fluorescence imagery for a wide range of quantitative, statistically robust image-based assays.
Learn more about ImageStream®X Mark II
Magnification 20X Fixed 60X / 40X / 20X
Pixel area 1.0 square microns  0.1 / 0.25 / 1.0 square microns
Number of Channels 12 6 or 12 high resolution
Max Field of View Width 64 microns 128 microns
Quantitative Image Analysis Standard Standard
Brightfield Illumination 10 Channels 10 Channels
488 nm Excitation Laser 60 mW 200 mW Standard, 400 mW Optional
Laser Power Doubler Standard Standard
375 nm Excitation Laser - 70 mW
405 nm Excitation Laser 100 mW 120 mW
561 nm Excitation Laser 50 mW 200 mW
592 nm Excitation Laser - 300 mW
642 nm Excitation Laser 100 mW 150 mW
730 nm Excitation Laser - 50 mW
785 nm Side Scatter Laser 70 mW 70 mW
Sample format microcentrifuge tube microcentrifuge tube
AutoSampler Optional Optional
Extended Depth of Field - Optional
Sensitivity 10 MESF 5 MESF