How it Works – Extended Depth of Field

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Figure 1 shows the effect of progressive defocus on a test pattern of 2μ bars.

Figure 2 shows how the same pattern imaged with EDF technology maintains excellent image quality over the entire focus range.
A unique optical system upgrade for the ImageStream®Xcytometer increases the depth of cell in focus without loss of fluorescence sensitivity. Now you can image FISH spots or other sub-cellular features no matter where they lie in the cell.

Image More of the Cell in Focus

The extended depth of field (EDF™) option for Amnis® imaging flow cytometry system incorporates Wavefront Coding™ technology from CDM Optics, which is a combination of specialized optics and unique image processing algorithms, to project more structures within the cell into one crisp plane of focus.

EDF™ technology dramatically extends the depth of field, enabling new applications and improving the performance of existing applications – especially spot counting applications such as fluorescence In-Situ Hybridization (FISH).