FlowSight® Imaging Flow Cytometer

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Amnis FlowSight Imaging Instrument.
The FlowSight® Imaging Flow Cytometer stands apart from other flow cytometers by producing up to 12 images of each and every cell. The unique image collection system simultaneously produces darkfield (side scatter), brightfield, and up to 10 fluorescence images. The FlowSight® Imaging Flow Cytometer operates at ~20X magnification allowing visualization of fluorescence from the membrane, cytoplasm, or nucleus. Identifying cell conjugates or distinguishing single cells from doublets and debris is effortless. Flow cytometry has never been so intuitive.

View an Overview Video on the FlowSight® Imaging Flow Cytometer

Amnis® brand FlowSight® Imaging Flow Cytometer – Flow Cytometry with Vision
FlowSight® imaging flow cytometry provides exceptional sensitivity as well as images of every cell in up to 10 fluorescent channels at up to 2,000 events per second. A proprietary CCD camera, coupled with patented detection technology, yields fluorescence sensitivity that is at least ten times that of PMT-based flow cytometers across all channels. View this informative video to learn more.

Capable: Sensitive and Flexible for Every Need

This imaging flow cytometer offers high performance in a small package. Its innovative design increases signal and minimizes noise to provide unmatched fluorescence sensitivity. Twelve standard detection channels simultaneously produce brightfield, darkfield and up to ten channels of fluorescence imagery of every cell. With these unique capabilities, the FlowSight® Imaging Flow Cytometer enables a broad range of applications.

Intuitive: Easy-to-use, With Imagery for Every Cell

The FlowSight® instrument operates like a conventional flow cytometer, but also provides imagery of every cell. Its powerful and intuitive analysis software seamlessly links quantitative data to imagery:
  • Click on a dot in any plot to see the corresponding cell imagery.
  • Click on a bin in any histogram to view all the cells in that bin.
  • Draw gates on dot plots and view the resulting populations to validate results.

Visual: Quantitative Imaging

Quantitative Imaging to maximize FlowSight®’s performance includes, sensitivity at 1 micron per pixel, flexibility to move brightfield channels, post-acquisition image processing software allows highly quantitative determinations of the location and strength of fluorescence signals for morphological applications such as nuclear translocation and shape change. IDEAS® Software combines image analysis, statistical rigor, and visual confirmation in an easy-to-use package.

Affordable: Designed and Priced for Every Lab

The FlowSight® Imaging Flow Cytometer is powerful enough for the core lab, but sized and priced for any lab. The system can be factory configured or field upgraded with up to four excitation lasers (405, 488, 561, 642 nm), a 96-well plate AutoSampler, and powerful quantitative image processing. Whether in a base configuration or fully-optioned, the FlowSight® instrument sets a new standard of value.