Distinguishing cells based on their morphologic differences is useful in the study of stem cell differentiation, hematology and oncology, and chemokine-induced shape change, just to name a few examples. Amnis® imaging flow cytometry provides numerous features that quantify cell size, shape and texture that are useful in morphologic classification. Combined with the large population sizes afforded by Amnis® imaging flow cytometry, even subtle changes in cell morphology can be quantified with statistical robustness.
Watch to learn how multispectral imaging in flow can use cellular morphology to enhance research in diverse cellular research contexts. Dr. Sherree Friend explains how Amnis® applications use high-throughput imaging of human PBMCs to characterize shape change in primary monocytes in response to inflammatory stimuli. Using the circularity feature, Dr. Friend explains how the IDEAS® software automatically calculates variance in cell radii to quantify response to MCP-1.