Antibodies for Cell Structure Research

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Cell structure research aims to link the structural features to mechanistic functions of a cell. A cell’s structural features, including the cytoskeleton, filaments, microtubules and adhesion molecules, enable cell migration, invasion, organelle transport, cell division, extracellular matrix degradation and restructuring, cell-cell communication and other functions. A thorough understanding of cell structure and function is essential for gaining deeper, systems-level elucidation of normal pathways such as morphogenesis, wound healing, neurogenesis and immune response, as well as abnormal processes such as metastasis, tumor-related angiogenesis and other disease-related events.

MilliporeSigma is the leading provider of cell structure research tools. Our offering includes our unique selection of cell adhesion, cytoskeleton, and ECM antibodies and proteins, our trusted and well-published Calbiochem inhibitors, and our quantitative, optimized cell-based assays. We are continuously expanding our comprehensive portfolio of innovative tools to keep our customers at the forefront of cell structure research.

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Cytoskeletal Structure
Cytoskeletal Structure
The cytoskeleton consists of actin, microtubules, and intermediate filaments (IFs). Actin polymers, which mediate cell movement and intracellular dynamics, are regulated by signaling proteins, including those of the N-WASP family.
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Microtubules, directing movement of subcellular components, are stabilized by Rho GTPases, microtubule-associated proteins, and organization centers. IFs are a diverse family of structural proteins with equally diverse stabilizing mechanisms. Molecular motor proteins move along actin filaments or microtubules and include myosins, kinesins, and dyneins. These proteins mediate the sliding of filaments and transport organelles along cytoskeletal tracks.

MilliporeSigma provides antibodies and kits for studying components of the cytoskeleton, including actin, vimentin, keratin, neurofilaments, lamin and tubulin, and also provides innovative solutions to investigate signaling proteins that associate with the cytoskeleton.
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Cell Adhesion
Cell Adhesion
Cell-to-cell and cell-to-extracellular matrix (ECM) adhesion enables cell communication and movement and is critical for the development and maintenance of tissues.
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Adhesion is involved in immune cell migration, metastasis, angiogenesis, wound healing, and other processes. Changes in cell adhesion can be the defining event in diseases such as cancer, osteoporosis, atherosclerosis, and arthritis. Adhesion between tumor cells and their microenvironment involves integrins, matrix metalloproteases (MMPs), and the ECM. Many interactions with the ECM are mediated by integrins, which transduce bidirectional signals controlling cell growth, differentiation, migration, apoptosis and more.

MilliporeSigma offers a wide selection of antibodies against integrins, and other cell-adhesion molecules (CAMs).
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Metalloproteinases and Proteases
Metalloproteinases and Proteases
Matrix metalloproteinases (MMPs) are secreted or transmembrane proteolytic endopeptidases that process and degrade extracellular matrix proteins.
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MMPs play critical roles in many normal growth and developmental aspects of tissue remodeling, wound healing, and angiogenesis. In a pathological context, MMPs are associated with cell migration, invasion, arthritis, and cancer tumor progression. Once activated, the MMPs are subject to inhibition by the tissue inhibitors of metalloproteinases (TIMPs) that bind MMPs non-covalently. ADAMs (A Disintegrin And Metalloprotease protein) are members of the same superfamily as MMPs and are cell surface proteins that possess both an adhesion domain as well as a protease domain.

MilliporeSigma offers a full range of MMP antibodies, proteins, and activity assays for interrogating the function and regulation of metalloproteinases (MMPs) in both normal and disease states.
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Cell Visualization
Cell Visualization
Cell visualization and microscopy has remained a fundamental tool for investigating cellular behavior and structure, including changes in structural features as well as cellular and sub-cellular dynamics under normal and diseased states.
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As the technology for microscopy has evolved, the ability to interrogate discrete protein and organelle dynamics has increased and the need for specific visualization tools has grown.

MilliporeSigma’s emerging portfolio of biosensors and cell-based assays provide innovative cell visualization and staining tools that complement our suite of fluorescently-conjugated primary and secondary antibodies.

 
 

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