Purification of mAbs and Related Proteins

Chromatographic Methods, Strategies
and Optimization

Three-day Course

Course Description

This hands-on course provides a fundamental understanding of chromatographic methods and strategies to purify monoclonal antibodies and related molecules. The combination of theoretical lectures, industrial case studies and experiments in the lab enables participants to gain a profound knowledge of biochromatography processes.

Catalog number: MANNHEIM02
Dates & Venue
Steinbeis Transfer Center at
Mannheim University of Applied Sciences

Paul-Wittsack-Str. 10
68163 Mannheim, Germany

Email: stz-tb@hs-mannheim.de

Course Content
  • Theory and application of Protein A, ion exchange and mixed-mode chromatography
  • Purification strategies for antibodies and related molecules
  • Case studies presented by experts from industry
  • Quality and safety aspects
Learning Goals
  • Designing robust chromatographic unit operations
  • Defining and optimizing suitable purification strategies
  • Evaluating the pros and cons of different techniques
  • Selecting analytical tools for process development
  • Understand and use all the tools needed to remove process impurities and contaminants
Who should attend?
  • Process development scientists
  • Chemical and biochemical engineers
  • R&D scientists and technicians
  • Professionals in areas related to antibody processing
What will you receive?
  • A comprehensive course documentation
  • Summary of experimental results
  • Certificate of participation
Course Schedule and Program
Day 1: Start 12:00 am
  • Registration and Welcome
  • Basic principles of antibody purification
    • Antibody structure
    • Protein A affinity chromatography
    • Antibody purification by ion-exchange chromatography
    • Static and dynamic binding capacities
  • Practical, part I
    • Capture of antibodies by Protein-A affinity chromatography
    • Ion-exchange chromatography for antibody purification
    • Determination of dynamic IgG binding capacities
    • Analysis and discussion of results
  • Dinner
Day 2: 8.30 am - 05:30 pm
  • Different chromatographic techniques for the purification of antibodies: Hydrophobic interaction chromatography, hydroxyapatite & mixed mode chromatography, size exclusion chromatography
  • Case studies:
    • Regulatory aspects in protein purification and impurities testing (Dr. Bettina Katterle)
  • Practical, part II:
    • Design of an antibody purification process
  • Purification strategies and sequencing of purification steps
  • Post-Protein-A – steps in antibody purification
  • Optimization of intermediate purification steps
  • Optimization of polishing steps
Day 3: 9.00 am - 04:00 pm
  • Case study:
    • Production of monoclonal antibodies ( Xavier Le Saoût)
  • Scale up in chromatography
  • Presentation and discussion of results
  • Course summary and final discussion
Enrollment limited to 8 participants
Due to the hands-on nature of this course, enrollment is limited to 8. We recommend early booking to ensure a place.
Price
€1050/person

For help with registration, hotel accommodation etc., please contact Mrs. Andrea Bentz, Assistant to Prof. Dr. C. Frech (Email: stz-tb@hs-mannheim.de).

For more information or to register for this course, please contact ilearn@emdmillipore.com

Course Instructors and Industry Experts
About our instructors

Our highly skilled and qualified instructors are experts in chromatography, and combine technical knowledge with field experience and teaching capabilities. The balance between theory and practice extends to our instructors who do not spend all their time in the theoretical environment of the classroom; they are also working engineers who deal with real applications on a daily basis.

Prof. Dr. Christian Frech, Steinbeis Transfer Centre at the University of Applied Sciences, Mannheim
  • 2004-present: Director of Steinbeis Transfer Center
  • Since 2004: Head of the Institute for Biochemistry at Mannheim University of Applied Sciences
  • March 2002: Appointment as Professor for Bioanalytics at Mannheim University of Applied Sciences
  • 1996–2002: Head of Pilot Plant Protein Purification, Process Development department at Chiron Behring, Marburg, Germany (now GSK Vaccines GmbH)
  • Post-doc at Boehringer Mannheim, Penzberg, Germany (now Roche Diagnostics)
Dr. Bettina Katterle, Head of Marketing & Sales, BSL BIOSERVICE Scientific Laboratories GmbH, Planegg, Germany
  • 2013-present: Head of Marketing & Sales, BSL BIOSERVICE Scientific Laboratories GmbH for biological safety and activity testing
  • 2009-2013: Sales Specialist Biopharmaceutical Services, Charles River Laboratories, Erkrath, Germany
  • 2002-2009: Scientific Officer (GMP) in Protein chemistry, NewLab GmbH, Erkrath, Germany
  • 2000-2002: Research Scientist at Max-Planck Institute for Bioinorganic Chemistry
  • Post-doc at Arrhenius Laboratories, Department of Molecular Biology, Stockholm, Sweden
Xavier Le Saoût, Associate Manager BioProcess Sciences, Merck KGaA, Darmstadt Germany Healthcare, Corsier sur Vevey, Switzerland

Xavier joined MilliporeSigma 13 years ago to work primarily in the downstream pilot-scale development group to support technical transfer activities to manufacturing scale. In 2007, he integrated a new team in charge of pre-formulation activities and process intermediate stability studies. In 2010, he took the head of this team and contributed to the process development of several Mabs from lab scale until technical transfers to manufacturing. Since 2015, he manages a team in charge of evaluating innovative DSP technologies to define next generation purification processes for MilliporeSigma.

Dr. Lothar Jacob, Consultant, Darmstadt, Germany
  • 2015-present: Consultant, Downstream Processing
  • 1992-2015: various Marketing roles within the Life Science Business of MilliporeSigma, focusing on Chromatography media development
  • 1987-1992: Laboratory Head Protein Purification, Max-Planck Research for Structural Molecular Biology at DESY, Hamburg, Germany
Dr. Thomas von Hirschheydt, Principal Scientist, Roche Diagnostics GmbH, Penzberg, Germany
  • 2007-present: Group Leader in Biologics Research -Downstream Process Research and Supply, Diagnostics GmbH, Penzberg, Germany
  • 2003-2007: Group Leader in Medicinal Chemistry and High Throughput Purification, Diagnostics GmbH, Penzberg, Germany
  • Post-doc in Medicinal Chemistry, Roche Diagnostics GmbH, Mannheim and Penzberg, Germany