Protein Concentration
Amicon® stirred cells

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Amicon stirred cells for large volume sample filtration.Amicon stirred cells provide high flow rates with solutions up to 10% macrosolute concentration and are capable of rapid concentration, or salt removal followed by concentration in the same unit. Complete product recoveries can generally be achieved using the diafiltration set-up as illustrated at the bottom of this page.
How Stirred Cells Work to Concentrate Protein
For protein concentration, gas pressure is applied directly to ultrafiltration cell. Solutes above the membrane's molecular weight (MW) cut-off are retained in cell, while water and solutes below the cut-off pass into the filtrate and out of cell.
Advantages
  • Gentle magnetic stirring minimizes concentration polarization and shear denaturation.
  • All stirred cells can be autoclaved.
  • Five different sizes to handle volumes from 3mL to 400mL
  • High flow rates with solutions up to 10% macrosolute concentration
Applications
  • Concentrate, diafilter, and exchange buffers for macromolecule solutions including proteins, enzymes, antibodies and viruses.
Available in five sizes:
Model Number
Min. Volume
Max. Volume
8003 0.075 mL 3 mL
8010 1.0 mL 10 mL
8050 2.5 mL 50 mL
8200 5.0 mL 200 mL
8400 10 mL 400 mL


How Stirred Cells Work to Concentrate Protein
Diafiltration set-up and operation with Amicon selector valve, stirred cell and accessory reservoir. 1 - Ultrafiltrate, 2 - Ultrafiltration equipment, 3 - Pressure supply, 4 - Selector valve, 5 - Inlet, 6 - Outlet, 7 - Feed liquid, 8 - Reservoir