ECM590 | Blood Vessel Staining Kit

Paraffin-Embedded human tonsil tissue section stained with Anti-von Willibrand Factor antibody
1 kit  sufficient for 100 sections
以订单确认为准 更多信息
您节约了 ()。
Request Pricing
目前缺货 目前缺货


      <a class="null" href="null">联系客户服务</a>




      Species Reactivity Key Applications Detection Methods
      H IH(P),IHC Chromogenic
      Catalogue Number
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      Description Blood Vessel Staining Kit
      Overview Reagents and materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections and when stored at 2-8ºC is stable up to the expiration date. ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.


      Angiogenesis and tumor-associated neovascularization are complex processes by which new blood vessels are formed from pre-existing vasculature. Several studies implicate tumor-associated blood vessel formation as a central part in the process of growth, invasion and metastasis of malignancies (Rak et al., 1995; Jahroudi & Greenberger, 1995; Saphir, 1997).

      The most widely used endothelial cell markers for studying angiogenesis/neovascularization are von Willebrand Factor (Factor VIII Related Antigen) and CD31 (PECAM-1). von Willebrand Factor is synthesized by endothelial cells, causing adhesion of platelets to injured vessel walls and functions as a carrier and stabilizer for coagulation of Factor VIII. CHEMICON's von Willebrand Factor antibody reacts specifically with the endothelial cells of blood vessels and is a useful marker for the identification of endothelial lineage in tumors. It is important to note that not all endothelial cells synthesize von Willebrand factor, therefore a small percentage of these vascular-rich tumors might not stain with this antibody. CD31 or platelet endothelial adhesion molecule (PECAM-1), is a type I integral membrane glycoprotein and a member of the immunoglobulin super family of cell surface receptors. It is strongly expressed by all endothelial cells and weakly by several types of leucocytes (Parums et al., 1990). According to several studies, staining of blood vessels with CD31 antibodies has shown to be suitable for the identification of angiogenesis in several types of malignancies such as lung (Giatromanolaki et al., 1996), breast (Horak et al., 1992) and colorectal cancer (Takebayashi et al., 1996). CHEMICON's monoclonal antibody to CD31 is very specific for the staining of vascular endothelial cells in normal and cancerous tissue.

      CHEMICON's Blood Vessel Staining Kit is a very sensitive immunohistochemical tool for the detection of vascular endothelial cells, containing both anti-von Willebrand Factor and anti-CD31 primary antibodies and is applicable to a variety of species. This immunoperoxidase kit provides a simple, all-in-one method for the staining of frozen and paraffin-embedded sections in human (both CD31 and VWF) or rat and mouse (VWF only) tissues
      Product Information
      Detection method
      Application Reagents & materials supplied in the Blood Vessel Staining Kit are sufficient for staining 100 tissue sections & when stored at 2-8ºC is stable up to the expiration date.
      Key Applications
      • Immunohistochemistry (Paraffin)
      • Immunohistochemistry
      Application Notes ECM590 detects vWF and CD31 in human paraffin embedded tissues. In rat or mouse tissues only the vWF antibody is effective in paraffin; the CD31 antibody does not react with rodent CD31.
      Biological Information
      Species Reactivity
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions Do not freeze or expose to elevated temperatures. Discard any remaining reagents after the expiration date. Do not store kit components or perform staining in strong light, such as direct sunlight.
      Packaging Information
      Material Size 1 kit
      Material Package sufficient for 100 sections
      Transport Information
      Supplemental Information




      参考 | 13 有货 | 选择所有参考

      Bioceramic-mediated trophic factor secretion by mesenchymal stem cells enhances in vitro endothelial cell persistence and in vivo angiogenesis.
      Jiawei He,Martin L Decaris,J Kent Leach
      Tissue engineering. Part A 18 2012

      Brain-derived neurotrophic factor promotes tumorigenesis via induction of neovascularization: implication in hepatocellular carcinoma.
      Chi-Tat Lam,Zhen-Fan Yang,Chi-Keung Lau,Ka-Ho Tam,Sheung-Tat Fan,Ronnie T P Poon
      Clinical cancer research : an official journal of the American Association for Cancer Research 17 2011

      Dopamine inhibits pulmonary edema through the VEGF-VEGFR2 axis in a murine model of acute lung injury.
      Vohra PK, Hoeppner LH, Sagar G, Dutta SK, Misra S, Hubmayr RD, Mukhopadhyay D
      American journal of physiology Lung cellular and molecular physiology 2011

      Histidine-rich glycoprotein modulates the anti-angiogenic effects of vasculostatin.
      Klenotic PA, Huang P, Palomo J, Kaur B, Van Meir EG, Vogelbaum MA, Febbraio M, Gladson CL, Silverstein RL
      The American journal of pathology 176 2039-50 Epub 2010 Feb 18 2010

      显示摘要 全文本文章
      Structural variants of biodegradable polyesterurethane in vivo evoke a cellular and angiogenic response that is dictated by architecture.
      Jerome A Henry, Krishna Burugapalli, Peter Neuenschwander, Abhay Pandit
      Acta biomaterialia 5 29-42 2009

      Angiogenesis: the unifying concept in cancer?
      Saphir, A
      J. Natl. Cancer Inst., 89: 1658-9 (1997) 1997

      Prognostic value of angiogenesis in operable non-small cell lung cancer.
      Giatromanolaki, A, et al.
      J. Pathol., 179: 80-8 (1996) 1996

      Angiogenesis as an unfavorable prognostic factor in human colorectal carcinoma.
      Takebayashi, Y, et al.
      Cancer, 78: 226-31 (1996) 1996

      Consequences of angiogenesis for tumor progression, metastasis and cancer therapy.
      Rak, J W, et al.
      Anticancer Drugs, 6: 3-18 (1995) 1995

      The role of endothelial cells in tumor invasion and metastasis.
      Jahroudi, N and Greenberger, J S
      J. Neurooncol., 23: 99-108 (1995) 1995



      EpiGRO™, EndoGRO™ and FibroGRO™ Reagents


      Blood Vessel Staining Kit - Data Sheet


      Blood Vessel Staining Kit - Data Sheet