71576 pTriEx™-6 3C/LIC Vector Kit - Novagen

71576
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      71576-3
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          Description
          OverviewThe pTriEx™-6 3C/LIC vector is a 3C/LIC version of pTriEx-6. The pTriEx-6 3C/LIC vector is uniquely designed to allow rapid characterization of target genes in multiple expression systems. With this vector, a single recombinant plasmid can be used to test expression in E. coli, insect, and mammalian cells. Transient mammalian expression is mediated by the CMV immediate early enhancer and promoter. For expression in insect cells, the pTriEx-6 3C/LIC vector contains flanking baculovirus sequences to permit the generation of recombinant baculoviruses using the BacVector® System or BacMagic™ Kits. In baculovirus-infected insect cells, expression is driven by the very late p10 promoter. Expression in E. coli is regulated by the tightly controlled T7lac promoter. The pTriEx-6 3C/LIC vector contains an N-terminal 8 aa Strep•Tag® II coding sequence followed by a HRV 3C protease cleavage site and a multiple cloning site, with the option to encode optional sequences for a C-terminal thrombin cleavage site and a His•Tag® (10-histidine) fusion.


          * Strep•Tag® technology for protein purification and detection is covered under US Patent No. 5,506,121, UK Patent No. 2,272,698 and French Patent No. 93 13 066. Strep•Tactin® is covered by US Patent No. 6,103,493. These products are provided only for research use. Information about licenses for commercial use is available from IBA GmbH, Rudolf-Wissell-Str. 28, D-37079 Goettingen, Germany.




          The pTriEx vector is covered by US Patent 6,589,783 owned by EMD Millipore Corporation or its Affiliates. This Product is sold for research use only. Any commercial use of the Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Further, the pTriEx vector allows for expression of target genes under control of the T7 promoter and is also covered by US Patent 5,693,489. This patent is licensed by EMD Millipore Corporation from Brookhaven National Labs for research use only. Commercial entities need to obtain a research use license from Brookhaven National Labs prior to use of this Product. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71576
          Brand Family Novagen®
          References
          Product Information
          1 µgpTriEx-6 3C/LIC Vector
          8 µl3C/LIC β-gal Control Insert
          25 UT4 DNA Polymerase (LIC-qualified)
          50 µl10X T4 DNA Polymerase Buffer
          100 µl100 mM DTT
          40 µl25 mM dATP
          50 µl25 mM EDTA
          1.5 mlNuclease-free Water
          22 × 50 µlNovaBlue GigaSingles™ Competent Cells
          2 × 0.2 mlOrigami™ B(DE3)pLacI Competent Cells
          5 × 2 mlSOC Medium
          10 µlTest Plasmid (ampicillin resistant)
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
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          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Insect Expression > Insect Expression Vectors & Kits
          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Mammalian Expression > Mammalian Expression Vectors & Kits
          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors