71238 pIEx™-2 DNA - Novagen

71238
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71238-3
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          Plastic ampoule 20 μg
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          Description
          OverviewThe pIEx™ vectors are designed for cloning and high-level expression of proteins in transiently transfected Spodoptera-derived insect cells. Transcription is driven by the AcNPV-derived hr5 enhancer and immediate early promoter, IE1. pIEx-2 contains N-terminal GST•Tag, His•Tag, and S•Tag coding sequences, and a C-terminal HSV•Tag coding sequence. N-terminal vector encoded sequences can be completely removed by cloning into the PshA I site and cleaving the fusion protein with enterokinase.
          pIEx-2 is also available as an Ek/LIC vector. The Ek/LIC vector is prepared for rapid directional cloning of PCR-amplified DNA. Using specifically designed primers for amplification, inserts can be efficiently cloned without the need for restriction enzyme digestion or ligation.

          For sequencing or PCR of recombinants in pIEx-2, refer to the table below to choose the appropriate primers.
          Vector5′ Primer3′ Primeralt 5′ Primer
          pIEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
          pIEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
          pIEx-3S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
          pIEx-4IE1 Promoter PrimerAS S•Tag 18mer Primer
          NA
          pIEx-5IE1 Promoter PrimerAS S•Tag 18mer Primer
          NA
          pBiEx-1S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
          T7 Promoter Primer
          pBiEx-2S•Tag 18mer PrimerAS HSV•Tag PrimerIE1 Promoter Primer
          T7 Promoter Primer
          pBiEx-3IE1 Promoter PrimerAS S•Tag 18mer PrimerT7 Promoter Primer





          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71238
          Brand Family Novagen®
          SynonymsInsectDirect™ System- pIEx™-2 DNA
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          Storage -20°C
          Do not freeze Ok to freeze
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