71335 pET-46 Ek/LIC Vector Kit - Novagen

71335
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      Overview

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          Plastic ampoule 20 rxn
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          Description
          OverviewThe pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Target genes are cloned in pET plasmids under control of strong bacteriophage T7 transcription and (optionally) translation signals; expression is induced by providing a source of T7 RNA polymerase in the host cell. T7 RNA polymerase is so selective and active that, when fully induced, almost all of the cell's resources are converted to target gene expression; the desired product can comprise more than 50% of the total cell protein a few hours after induction. All of the pET vectors and companion products are available as kits designed for convenient cloning, expression, detection, and purification of target proteins.
          The pET-46 Ek/LIC Vector is a LIC-compatible derivative of pET-45b(+). The plasmid contains a strong T7lac promoter and an amino-terminal His•Tag® coding sequence immediately followed by an Ek/LIC cloning site designed to allow the generation of fusion proteins with minimal vector-encoded sequence. An optional C-terminal S•Tag™ coding sequence is compatible with purification, detection, and quantification (1). This plasmid is compatible with the LIC Duet™ Adaptors for coexpression of two proteins as well as with pCDF-2 and pRSF-2 Ek/LIC vectors for coexpression of up to six proteins when using the LIC Duet Adaptors.




          Commercial use of this Product requires a commercial license from EMD Millipore Corporation. Commercial use shall include but not be limited to (1) use of the Product or its components in manufacturing; (2) use of the Product or its components to provide a service, information, or data to others in exchange for consideration; (3) use of the Product or its components (or any derivatives thereof) for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the Product or its components, whether or not such Product or its components are resold for use in research. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of this Product.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71335
          Brand Family Novagen®
          References
          References1. Kim, J.S. and Raines, R.T. 1993. Protein Science 2, 348.
          Product Information
          1 µgEk/LIC Vector
          25 unitsT4 DNA Polymerase, LIC-qualified
          50 µl25 mM EDTA
          40 µl25 mM dATP
          8 µlEk/LIC β-gal Control Insert
          100 µl100 mM DTT
          50 µl10X T4 DNA Polymerase Buffer
          1.5 mlNuclease-free Water
          5 × 2 mlSOC Medium
          10 µlTest Plasmid
          0.2 mlBL21(DE3)pLysS Competent Cells
          0.2 mlBL21(DE3) Competent Cells
          22 × 50 µlNovaBlue GigaSingles™ Competent Cells
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pET-46 Ek/LIC Vector Kit - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pET-46 Ek/LIC Vector Kit - Novagen Certificates of Analysis

          TitleLot Number
          71335

          References

          Reference overview
          1. Kim, J.S. and Raines, R.T. 1993. Protein Science 2, 348.

          Citations

          Title
        • Park, M-O., et al. 2007. Journal of Bacteriology 189, 7281.
        • Smialowski, P., et al. 2007. Bioinformatics 23, 2536.
        • Yu, Z., et al. 2007. Nucleic Acids Research 35, 2107.
        • User Protocols

          Title
          TB055 pET System Manual
          TB163 Ek/LIC Cloning Kits
          TB184 Xa/LIC Cloning Kits

          Vector Map

          Title
          TB397VM pET-46 Ek/LIC Vector Map

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          Categories

          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors