71340 pCDFDuet™-1 DNA - Novagen

71340
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71340-3
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          Plastic ampoule 10 μg
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          Description
          OverviewpCDFDuet™-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is preceded by a T7 promoter, lac operator, and ribosome binding site (rbs). The vector also carries the pCloDF13 replicon, lacI gene and streptomycin/spectinomycin resistance marker. This vector can be transformed into the same cell with pETDuet™-1, pACYCDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 for the coexpression of up to eight target genes. ORFs inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. ORFs inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number71340
          Brand Family Novagen®
          Application Data

          LaneM: Trail Mix™ Protein Markers; Lane1: pACYDuet-1:β-gal + Fluc; Lane2: pETDuet-1:GST/GUS + GFP; Lane3: pRSFDuet-1:Nus/hlFNγ + S•Tag/T4 PNK; Lane4: pCDFDuet-1:GUS + His•Tag/MBP; Lane5: pACYDuet-1, pETDuet-1 combination; Lane6: pACYDuet-1, pRSFDuet-1 combination; Lane7: pACYDuet-1, pCDFDuet-1 combination; Lane8: pETDuet-1, pRSFDuet-1 combination; Lane9: pRSFDuet-1, pCDFDuet-1 combination; Lane10: pETDuet-1, pCDFDuet-1 combination; Lane11: pACYDuet-1, pETDuet-1, pRSFDuet-1 combination; Lane12: pACYDuet-1, pETDuet-1, pCDFDuet-1 combination; Lane13: pACYDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane14: pETDuet-1, pRSFDuet-1, pCDFDuet-1 combination; Lane15: Uninduced control; Lane16: All four Duet vectors: all eight proteins

          The indicated constructs were transformed individually or together into BL21(DE3). Cultures were grown in TB plus phosphates plus glucose at 37°C to and Abs600 between 1.0 and 1.2. Target protein expression was induced by adding IPTG to a final concentration of 1 mM. Cultures were harvested by centrifugation 2.5 h after induction. Lysates were produced by sonication using equal volumes of 1% SDS and 2X SDS sample buffer. Equivalent amounts of protein (based on harvest ABS) were analyzed by SDS-PAGE (4-20% gradient gel) and stained with coomassie blue. To maximize band separation, proteins smaller than 25 kDa were allowed to migrate off the gel.

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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
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          pCDFDuet™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pCDFDuet™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          71340

          Citations

          Title
        • Louis B. Rice, et al. (2007) Interaction of related Tn916-like transposons: analysis of excision events promoted by Tn916 and Tn5386 integrases. Journal of Bacteriology 189, 3909-3917.
        • Mary Anne T. Rubio, et al. (2007) An adenosine-to-inosine tRNA-editing enzyme that can perform C-to-U deamination of DNA. Procedings of the National Academy of Science 104, 7821-7826.
        • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
        • Wonduck Kim and F. Robert Tabita. (2006) Both subunits of ATP-citrate lyase from Chlorobium tepidum contribute to catalytic activity. Journal of Bacteriology 188, 6544-6552.
        • Zhizhong Li, et al. (2006) Structure of a BMI-1-ring1B polycomb group ubiquitin ligase complex. Journal of Biological Chemistry 281, 20643-20649.
        • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
        • Matthew A. Young, et al. (2006) Structure of the kinase domain of an imatinib-resistant Abl mutant in complex with the aurora kinase inhibitor VX-680. Cancer Research 66, 1007-1014.
        • Kai-Hong Zhao, et al. (2006) Chromophore attachment to phycobiliprotein -subunits: phycocyanobilin:cysteine-84 phycobiliprotein lyase activity of Cpes-like protein from Anabaena SP. PCC7120. Journal of Biological Chemistry 281, 8573-8581.
        • Melissa L. Geddie, et al. (2005) Rational design of p53, an intrinsically unstructured protein, for the fabrication of novel molecular sensors. Journal of Biological Chemistry 280, 35641-35646.
        • Markus Raschle, et al. (2004) Multiple interactions with the Rad51 recombinase govern the homologous recombination function of Rad54. Journal of Biological Chemistry (sept 2004) e copy,.
        • User Protocols

          Title
          TB340 Duet Vectors
          TB401 pRSF and pCDF Vectors

          Vector Map

          Title
          TB390VM pCDFDuet™-1 Vector Map

          Vector Sequence

          Title
          pCDFDuet™-1 Vector Sequence