70045 pBAC™4x-1 DNA - Novagen

70045
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      70045-3
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          Plastic ampoule 10 μg
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          Description
          OverviewpBAC4x-1 is a baculovirus transfer plasmid designed for cloning and co-expression of up to four target genes or multiple copies of the same gene in insect cells. The plasmid contains two polh promoters and two p10 promoters, each of which is upstream of unique cloning sites for sequential insertion of target genes. The homologous promoters are in opposite orientations to minimize recombination. The plasmid is compatible with BacVector™-1000, -2000 or -3000 Triple Cut Virus DNA for low background transfection and efficient utilization of the promoters. The vector provides optional expression of a C-terminal His•Tag® fusion sequence from the polh right-hand promoter by allowing read-through of inserts in the proper reading frame. Unique restriction sites are indicated on the circle map. The cloning/expression region of the coding strand transcribed from the clockwise promoters is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single stranded DNA that corresponds to this strand. Single stranded sequencing of phage-derived DNA can be performed using the 1629DWN primer.





          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70045
          Brand Family Novagen®
          References
          Product Information
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          Biological Information
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          Materials Information
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          Safety Information according to GHS
          Safety Information
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          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
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          pBAC™4x-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pBAC™4x-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          70045

          Citations

          Title
        • Junmin Peng, Nick F. Marshall and David H. Price. (1998) Identification of a cyclin subunit required for the function of Drosophila P-TEFb. Journal of Biological Chemistry 273, 13855-13860.
        • Vector Map

          Title
          TB133 pBAC4x-1 Vector Map

          Vector Sequence

          Title
          pBAC4x-1 Sequence