71147 pACYCDuet™-1 DNA - Novagen

71147
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      71147-3
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          Glass bottle 10 μg
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          Description
          OverviewCoexpression of multiple target genes in E. coli is advantageous for studying protein complexes. Coexpression often achieves optimal yield, solubility, and activity and may protect individual subunits from degradation. The Duet vectors are T7 promoter expression vectors, each designed to coexpress two target proteins in E. coli. The Duet vectors carry compatible replicons and antibiotic resistance markers and may be used together in appropriate host strains to coexpress up to eight proteins. Certain combinations of Duet vectors and pET or pETcoco™ vectors are also compatible for coexpression. The capability of Duet vectors to be cotransformed, propagated, and induced for robust target protein coexpression makes them ideal for the analysis of protein complexes. The Duet vectors are designed with compatible replicons and drug resistance genes for effective propagation and maintenance of four plasmids in a single cell.
          pACYCDuet-1 is designed for the coexpression of two target genes. The vector encodes two multiple cloning sites (MCS) each of which is receded by a T7 promoter, lac operator and ribosome binding site (rbs). The vector also carries the P15A replicon, lacI gene and chloramphenicol resistance gene. This vector can be transformed into the same cell with pETDuet™-1, pCDFDuet™-1, and pRSFDuet™-1 or pCOLADuet™-1 in the appropriate host strain for the coexpression of up to eight target genes. Genes inserted into MCS-1 can be sequenced using the ACYCDuetUP1 Primer and DuetDOWN1 Primer. Genes inserted into MCS-2 can be sequenced using the DuetUP2 Primer and T7 Terminator Primer.






          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
          Catalogue Number71147
          Brand Family Novagen®
          References
          Product Information
          Fusion tagHis•Tag, S•Tag
          Vector familyDuet
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage ≤ -70°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          pACYCDuet™-1 DNA - Novagen SDS

          Title

          Safety Data Sheet (SDS) 

          pACYCDuet™-1 DNA - Novagen Certificates of Analysis

          TitleLot Number
          71147

          Citations

          Title
        • Ee L. Ang, Jeffrey P. Obbard and Huimin Zhao. (2007) Probing the molecular determinants of aniline dioxygenase substrate specificity by saturation mutagenesis. Federation of American Societies for Experimental Biology Journal 274, 928-939.
        • Daphna Frenkiel-Krispin, et al. (2007) Plant transformation by Agrobacterium tumefaciens: modulation of single-stranded DNA-Vir2 complex assembly by VirE1. Journal of Biological Chemistry 282, 3458-3464.
        • Jun Li, et al. (2007) The two active sites in human branched-chain α-keto acid dehydrogenase operate independently without an obligatory alternating-site mechanism. Journal of Biological Chemistry 282, 11904-11913.
        • W. David Tolbert, et al. (2007) A mechanistic basis for converting a receptor tyrosine kinase agonist to an antagonist. Procedings of the National Academy of Science 104, 14592-14597.
        • Meimei Xu, P. Ross Wilderman and Reuben J. Peters. (2007) Following evolution's lead to a single residue switch for diterpene synthase product outcome. Procedings of the National Academy of Science 104, 7397-7401.
        • Kai-Hong Zhao, et al. (2007) Phycobilin:cystein-84 biliprotein lyase, a near-universal lyase for cysteine-84-binding sites in cyanobacterial phycobiliproteins. Procedings of the National Academy of Science 104, 14300-14305.
        • Jules Beekwilder, et al. (2006) Production of resveratrol in recombinant microorganisms. Applied and Enviornmental Microbiology 72, 5670-5672.
        • Niraj H. Tolia and Leemor Joshua-Tor. (2006) Strategies for protein coexpression in Escherichia coli. Nature Methods 3, 55-64.
        • Yu-hang Chen, et al. (2004) Structural basis of the α1-β subunit interaction of voltage-gated Ca2+ channels. 429, 675-680.
        • Yuanhe Li, et al. (2004) Structural basis for L27 domain-mediated assembly of signaling and cell polarity complexes. European Molecular Biology Organization Journal 23, 2723-2733.
        • Matthias Versele and Jeremy Thorner. (2004) Septin collar formation in budding yeast requires GTP binding and direct phosphorylation by the PAK, Cla4. Journal of Cell Biology 164, 701-715.
        • Matthias Versele, et al. (2004) Protein-rotein interactions governing septin heteropentamer assembly and septin filament organization in Saccharomyces cerevisiae. Molecular Biology of the Cell 15, 4568-4583.
        • Benedykt Wladyka, Katarzyna Puzia and Adam Dubin. (2004) Efficient co-expression of a recombinant staphopain A and its inhibitor staphostatin A in Escherichia coli. Biochemical Journal.
        • User Protocols

          Title
          TB340 Duet Vectors

          Vector Map

          Title
          TB336VM pACYCDuet™-1 Vector Map

          Vector Sequence

          Title
          pACYCDuet-1 Vector Sequence