506165 | p53, His•Tag®, Human, Recombinant, E. coli

506165
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      506165-10UG
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      Stocked 
      Discontinued
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      Available
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          Plastic ampoule 10 μg
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          Description
          OverviewRecombinant, human, wild-type p53 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.
          Catalogue Number506165
          Brand Family Calbiochem®
          SynonymsTumor Protein p53
          References
          ReferencesLiu, G., et al. 2003. J. Biol. Chem. 278, 17557.
          Zhang, L., et al. 2000. Cancer Res. 60, 3655.
          Fields, S., and Jang, S.K. 1990. Science 249, 1046.
          Michalovitz, D., et al. 1990. Cell 62, 671.
          Raycroft, L., et al. 1990. Science 249, 1049.
          Finlay, C.A., et al. 1989. Cell 57, 1083.
          Product Information
          FormLiquid
          FormulationIn 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.
          Applications
          Biological Information
          Biological activityTypical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.
          Purity≥80% by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          ContaminantsProteases: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          506165

          References

          Reference overview
          Liu, G., et al. 2003. J. Biol. Chem. 278, 17557.
          Zhang, L., et al. 2000. Cancer Res. 60, 3655.
          Fields, S., and Jang, S.K. 1990. Science 249, 1046.
          Michalovitz, D., et al. 1990. Cell 62, 671.
          Raycroft, L., et al. 1990. Science 249, 1049.
          Finlay, C.A., et al. 1989. Cell 57, 1083.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision19-March-2009 JSW
          SynonymsTumor Protein p53
          DescriptionRecombinant, human, wild-type p53 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. p53, a tumor suppressor, blocks transformation and inhibits tumor cell growth. Numerous mutations in the p53 gene have been characterized in human cancers. Loss or mutation of p53 function is highly correlated with tumorigenesis. The purified protein is useful in DNA binding assays and as a protein kinase substrate.
          FormLiquid
          FormulationIn 50 mM KCl, 30 mM sodium pyrophosphate, 5% glycerol, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥80% by SDS-PAGE
          ContaminantsProteases: none detected
          Biological activityTypical DNA binding assays require 0.5 ng to 2 ng of p53 protein per assay.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesLiu, G., et al. 2003. J. Biol. Chem. 278, 17557.
          Zhang, L., et al. 2000. Cancer Res. 60, 3655.
          Fields, S., and Jang, S.K. 1990. Science 249, 1046.
          Michalovitz, D., et al. 1990. Cell 62, 671.
          Raycroft, L., et al. 1990. Science 249, 1049.
          Finlay, C.A., et al. 1989. Cell 57, 1083.