506114 p34cdk1/Cyclin B, Human, Recombinant, S. frugiperda

506114
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      506114-200U
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      Stocked 
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          Plastic ampoule 200 u
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          Description
          OverviewRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB).
          Catalogue Number506114
          Brand Family Calbiochem®
          References
          ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
          Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
          Product Information
          Activity≥20,000 U/ml
          Unit of DefinitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
          Applications
          Biological Information
          Physicochemical Information
          ContaminantsProtease, phosphatase: none detected
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          506114

          References

          Reference overview
          Kobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
          Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-August-2008 RFH
          DescriptionRecombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, p34cdc2 is inactive and requires association with cyclin B to form an active complex.
          FormLiquid
          FormulationIn 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5.
          Recommended reaction conditions50 mM Tris-HCl, 10 mM MgCl2, 2 mM DTT, 1 mM EGTA, 100 µM ATP, 0.01% BRIJ® 35 detergent, pH 7.5.
          ContaminantsProtease, phosphatase: none detected
          Activity≥20,000 U/ml
          Unit definitionOne unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesKobayashi, H., et al. 1994. J. Biol. Chem. 269, 29153.
          Atherton-Fessler, S., et al. 1993. Mol. Cell. Biol. 13, 1675.