484235 | p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem

484235
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₄₀H₃₀Cl₂N₁₀O₆ 298-83-9

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      484235-1GM
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          Glass bottle 1 gm
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          484235-250MG
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              Glass bottle 250 mg
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              Description
              OverviewNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
              Catalogue Number484235
              Brand Family Calbiochem®
              SynonymsNBT, Nitro BT
              References
              ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
              De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
              Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
              Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
              Nichols, K., et al. 1992. Neuroscience 51, 791.
              Nichols, K., et al. 1992. Neuroscience 51, 791.
              Product Information
              CAS number298-83-9
              FormYellow crystalline solid
              Hill FormulaC₄₀H₃₀Cl₂N₁₀O₆
              Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
              Hygroscopic Hygroscopic
              Structure formula ImageStructure formula Image
              Applications
              Applicationp-Nitroblue Tetrazolium Chloride, CAS 298-83-9, is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions.
              Biological Information
              Primary TargetNOS
              Primary Target IC<sub>50</sub>3-4 µM
              Purity≥98% by TLC
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSXF8045000
              Safety Information
              R PhraseR: 20/21/22-68

              Harmful by inhalation, in contact with skin and if swallowed.
              Possible risks of irreversible effects.
              S PhraseS: 22-26-36/37

              Do not breathe dust.
              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing and gloves.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage +2°C to +8°C
              Protect from Light Protect from light
              Hygroscopic Hygroscopic
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              484235

              References

              Reference overview
              Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
              De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
              Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
              Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
              Nichols, K., et al. 1992. Neuroscience 51, 791.
              Nichols, K., et al. 1992. Neuroscience 51, 791.

              Brochure

              Title
              Enzyme-Linked Detection Systems Technical Bulletin
              Nitric Oxide and Oxidative Stress Brochure
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision13-August-2008 RFH
              SynonymsNBT, Nitro BT
              DescriptionNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
              FormYellow crystalline solid
              CAS number298-83-9
              RTECSXF8045000
              Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
              Structure formulaStructure formula
              Purity≥98% by TLC
              SolubilityMeOH or H₂O
              Storage +2°C to +8°C
              Hygroscopic
              Protect from light
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
              Toxicity Harmful
              ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
              De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
              Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
              Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
              Nichols, K., et al. 1992. Neuroscience 51, 791.
              Nichols, K., et al. 1992. Neuroscience 51, 791.