324355 n-Dodecyl-β-D-maltoside, ULTROL® Grade - CAS 69227-93-6 - Calbiochem

324355
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      Overview

      Replacement Information

      Key Specifications Table

      CAS #
      69227-93-6

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      324355-100GM
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          Glass bottle 100 gm
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          324355-1GM
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              Glass bottle 1 gm
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              324355-250GM
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                  Glass bottle 250 gm
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                  324355-25GM
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                      Glass bottle 25 gm
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                      324355-5GM
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                          Plastic ampoule 5 gm
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                          Description
                          OverviewNon-ionic detergent similar to n-octyl-β-D-glucopyranoside; however, increased alkyl chain length has been shown to increase thermal stability of lipid-free rhodopsin in solution. Useful in membrane-protein solubilization studies. Aggregation number: 98.
                          Absorbance (10%, H2O, 260 nm): <1.0.
                          Catalogue Number324355
                          Brand Family Calbiochem®
                          References
                          ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
                          Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
                          Rosevear, P., et al. 1980. Biochemistry 19, 4108.
                          Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
                          Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
                          Product Information
                          CAS number69227-93-6
                          Aggregation Number98
                          CMC100-600 µM
                          FormWhite solid
                          Hygroscopic Hygroscopic
                          Micellar weight50000
                          Structure formula ImageStructure formula Image
                          Applications
                          Biological Information
                          Purity≥98% by HPLC
                          Physicochemical Information
                          Contaminantsdodecanol 1H NMR: ≤1%
                          Dimensions
                          Materials Information
                          Toxicological Information
                          Safety Information according to GHS
                          Safety Information
                          Product Usage Statements
                          Storage and Shipping Information
                          Ship Code Ambient Temperature Only
                          Toxicity Standard Handling
                          Storage +2°C to +8°C
                          Hygroscopic Hygroscopic
                          Do not freeze Ok to freeze
                          Special InstructionsFollowing reconstitution, refrigerate (4°C).
                          Stock solutions are stable for up to 3 months at 4°C.
                          Packaging Information
                          Transport Information
                          Supplemental Information
                          Specifications

                          Documentation

                          SDS

                          Title

                          Safety Data Sheet (SDS) 

                          Certificates of Analysis

                          TitleLot Number
                          324355

                          References

                          Reference overview
                          Witzmann, F., et al. 1991. Electrophoresis 12, 687.
                          Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
                          Rosevear, P., et al. 1980. Biochemistry 19, 4108.
                          Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
                          Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.

                          Brochure

                          Title
                          CALBIOSORBル Technical Bulletin
                          Detergents and Buffers Technical Bulletin

                          Citations

                          Title
                        • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.
                        • Data Sheet

                          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

                          Revision20-December-2010 JSW
                          DescriptionA water-soluble nonionic detergent. Studies on rhodopsin have shown that alkyl glycosides with longer alkyl chains are better at preserving the protein's properties. In addition, dodecyl maltoside's longer hydrophilic group confers higher solubility in water. This is in contrast to undecyl and dodecyl glucosides, which have low solubility in water. Due to its low CMC, dodecyl maltoside is not easily removed by dialysis. The half time for dialysis is 20 h. This detergent is milder than nonyl glucoside. Of several glycosides tested, dodecyl maltoside was found to be the most useful for the preparation of active cytochrome oxidase from Neurospora. In octyl glucoside, cytochrome oxidase dissociated into its subunits and became inactive. Similarly, beef heart oxidase when equilibrated in octyl glucoside formed a mixture of polymer, dimer, and lower molecular weight forms and underwent denaturation. In contrast, the enzyme, when equilibrated in dodecyl maltoside, was found to exist as a uniform species of 30 kDa, which is consistent with an active dimeric form. Dodecyl maltoside has a critical micelle concentration (CMC) of 100-600 µM and a micellar weight of 70,000. Aggregation number: 98.
                          FormWhite solid
                          CAS number69227-93-6
                          Structure formulaStructure formula
                          Purity≥98% by HPLC
                          Contaminantsdodecanol 1H NMR: ≤1%
                          SolubilityH₂O (100 mg/ml)
                          Storage +2°C to +8°C
                          Hygroscopic
                          Do Not Freeze Ok to freeze
                          Special InstructionsFollowing reconstitution, refrigerate (4°C).
                          Stock solutions are stable for up to 3 months at 4°C.
                          Toxicity Standard Handling
                          ReferencesWitzmann, F., et al. 1991. Electrophoresis 12, 687.
                          Bass, W.T., and Bricker, T.M. 1988. Anal. Biochem. 171, 330.
                          Rosevear, P., et al. 1980. Biochemistry 19, 4108.
                          Grip, W.J., and Bovee-Geurts, P.H.M. 1979. Chem. Phys. Lipids 23, 321.
                          Stubbs, G.W., et al. 1976. Biochim. Biophys. Acta 425, 46.
                          Citation
                        • Serban P. Georgescu, et al. (2005) Modulator recognition factor 1 (MRF1), an ARID-family member, is a novel co-repressor for estrogen receptor α. Molecular Endocrinology 19, 2491-2501.