71186 YeastBuster™ Protein Extraction Reagent

71186
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71186-3
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          Plastic ampoule 100 ml
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          71186-4
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              Plastic ampoule 500 ml
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              Description
              OverviewYeastBuster™ Protein Extraction Reagent is formulated for a fast, efficient and gentle extraction of active proteins from yeast cells. The reagent avoids harsh conditions of vigorous mechanical or chemical treatment that often result in heat degradation of target proteins. The proprietary formulation utilizes a mix of mild detergent, protein stabilization buffer, and tris(hydroxypropyl) phosphine (THP) reducing agent (THP concentrate provided separately). This powerful combination eliminates the inconsistencies associated with tedious mechanical disruption of yeast cells with glass bead abrasives, ultrasonication and pressure disruption, or enzymatic digestion with β-1,3-glucanase lytic enzymes. The reagent has been tested with Saccharomyces cerevisiae, Pichia pastoris, P. stipidis, and Schizosaccharomyces pombe strains and with plant cells.

              In practice, cells are harvested by centrifugation and suspended in YeastBuster. Following a brief incubation, insoluble cell debris is removed by centrifugation, and the clarified extract is ready to use. In addition to greater total protein yields in crude extracts and recovery of enzymatically active protein, the extracts are fully compatible with GST•Bind™ and Ni-NTA His•Bind® immobilized metal affinity chromatography (IMAC) purification methods. The reagent is available in 100- and 500-ml sizes.








              Catalogue Number71186
              Brand Family Novagen®
              Features and benefits
              • Gentle, rapid, efficient extraction of proteins from yeast cells
              • Eliminates the inconsistencies associated with abrasive grinding, ultrasonication, and pressure disruption of yeast cells
              • Higher yield of total and enzymatically active proteins
              • Fully compatible with Ni-NTA His•Bind® and GST•Bind™ affinity purification methods
              References
              Product Information
              Components
              Applications
              Biological Information
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Multiple Toxicity Values, refer to MSDS
              Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
              Storage Multiple storage requirements
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              YeastBuster™ Protein Extraction Reagent Certificates of Analysis

              TitleLot Number
              71186

              Technical Info

              Title
              TB316 YeastBuster Protein Extraction Reagent

              Citations

              Title
            • Jennifer A. Gibbons, et al. (2007) Expression of human protein phosphatase-1 in Saccharomyces cerevisiae highlights the role of phosphatase isoforms in regulating eukaryotic functions. Journal of Biological Chemistry 282, 21838-21847.
            • Alyssa Dilla, et al. (2004) The Arabidopsis F-Box protein SLEEPY1 targets gibberellin signaling repressors for gibberellin-induced degradation. The Plant Cell 16, 1392-1405.
            • User Protocols

              Title
              TB316 YeastBuster™ Extraction Reagent