681675 Wortmannin - CAS 19545-26-7 - Calbiochem

681675
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₃H₂₄O₈ 19545-26-7

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      681675-1MG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Glass bottle 1 mg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewA cell-permeable, fungal metabolite that acts as a potent, selective, and irreversible inhibitor of phosphatidylinositol 3-kinase in purified preparations and cytosolic fractions (IC50 = 5 nM). Blocks the catalytic activity of PI 3-kinase without affecting the upstream signaling events. Preincubation of fibroblasts with wortmannin abolishes PDGF-mediated Ins(3,4,5)P3 formation (IC50 = 5 nM). Also blocks the metabolic effects of insulin in isolated rat adipocytes without affecting the insulin receptor tyrosine kinase activity. Inhibits MAP kinase activation induced by platelet activating factor in guinea pig neutrophils (200 - 300 nM). Also inhibits other kinases such as myosin light chain kinase (IC50 = 200 nM) and PI 4-kinase at concentrations higher than that required for inhibition of PI 3-kinase. Also blocks phospholipase D. A 10 mM (1 mg/233 µl) solution of Wortmannin (Cat. No. 681676) in DMSO is also available.
          Catalogue Number681675
          Brand Family Calbiochem®
          SynonymsKY 12420, MLCK Inhibitor II
          References
          ReferencesCross, M.J., et al. 1995. J. Biol. Chem. 270, 25352.
          Nakamura, I., et al. 1995. FEBS Lett. 361, 79.
          Ferby, I.M., et al. 1994. J. Biol. Chem. 269, 30485.
          Okada, T., et al. 1994. J. Biol. Chem. 269, 3568.
          Wymann, M.P. and Arcaro, A. 1994. Biochem. J. 298, 517.
          Arcaro, A. and Wymann, M.P. 1993. Biochem. J. 296, 297.
          Nakanishi, S., et al. 1992. J. Biol. Chem. 267, 2157.
          Bonser, R.W., et al. 1991. Br. J. Pharmacol. 103, 1237.
          Product Information
          CAS number19545-26-7
          ATP CompetitiveN
          FormWhite to off-white solid
          Hill FormulaC₂₃H₂₄O₈
          Chemical formulaC₂₃H₂₄O₈
          ReversibleN
          Structure formula ImageStructure formula Image
          Applications
          Biological Information
          Primary Targetphosphatidylinositol-3-kinase
          Primary Target IC<sub>50</sub>5 nM against phosphatidylinositol 3-kinase; 5 nM against PDGF-mediated Ins(3,4,5)P3 formation; 200 nM against myosin light chain kinase
          Purity≥98% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSCB9641000
          Safety Information
          R PhraseR: 26/27/28

          Very toxic by inhalation, in contact with skin and if swallowed.
          S PhraseS: 22-36/37/39-45

          Do not breathe dust.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Highly Toxic
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Wortmannin - CAS 19545-26-7 - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Wortmannin - CAS 19545-26-7 - Calbiochem Certificates of Analysis

          TitleLot Number
          681675

          References

          Reference overview
          Cross, M.J., et al. 1995. J. Biol. Chem. 270, 25352.
          Nakamura, I., et al. 1995. FEBS Lett. 361, 79.
          Ferby, I.M., et al. 1994. J. Biol. Chem. 269, 30485.
          Okada, T., et al. 1994. J. Biol. Chem. 269, 3568.
          Wymann, M.P. and Arcaro, A. 1994. Biochem. J. 298, 517.
          Arcaro, A. and Wymann, M.P. 1993. Biochem. J. 296, 297.
          Nakanishi, S., et al. 1992. J. Biol. Chem. 267, 2157.
          Bonser, R.W., et al. 1991. Br. J. Pharmacol. 103, 1237.

          Citations

          Title
        • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
        • Nadine N. Johnson-Farley, Tatyana Travkina and Daniel S. Cowen. (2005) Cumulative activatino of Akt and consequent inhibition of glycogen synthase kinase-3 by brain-derived neurotrophic factor and insulin-like growth factor-1 in cultured hippocampal neurons. Journal of Pharmacology and Experimental Therapeutics in press,.
        • Xu-Wen Liu, et al. (2005) Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1. Cancer Research 65, 898-906.
        • Kuntebommanahalli N. Thimmaiah, et al. (2005) Identification of N10-Substituted Phenoxazines as Potent and Specific Inhibitors of Akt Signaling. Journal of Biological Chemistry in press,.
        • Yung-Luen Yu, et al. (2005) MAP kinase-mediated phosphorylation of GATA-1 promotes Bcl-XL expression and cell survival. Journal of Biological Chemistry 280, 29533-29542.
        • Hui Zeng, et al. (2005) Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. American Journal of Physiology Gastrointestinal and Liver Physiology in press,.
        • Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-March-2019 JSW
          SynonymsKY 12420, MLCK Inhibitor II
          DescriptionA fungal metabolite that acts as a potent, selective, cell-permeable and irreversible inhibitor of phosphatidylinositol 3-kinase (PI 3-kinase) in purified preparations and cytosolic fractions (IC50 = 5 nM). Preincubation of fibroblasts with wortmannin abolishes PDGF-mediated Ins(3,4,5)P3 formation (IC50 = 5 nM). Blocks the catalytic activity of PI 3-kinase without affecting the upstream signaling events. Also blocks the metabolic effects of insulin in isolated rat adipocytes without affecting the insulin receptor tyrosine kinase activity. Inhibits MAP kinase activation induced by platelet activating factor in guinea pig neutrophils (0.2-0.3 µM). Inhibits osteoclastic bone resorption. Inhibits other kinases, such as myosin light chain kinase (IC50 = 200 nM) and PI 4-kinase at concentrations 100-fold higher than that required for the inhibition of PI 3-kinase. Also blocks phospholipase D.
          FormWhite to off-white solid
          Intert gas (Yes/No) Packaged under inert gas
          CAS number19545-26-7
          RTECSCB9641000
          Chemical formulaC₂₃H₂₄O₈
          Structure formulaStructure formula
          Purity≥98% by HPLC
          SolubilityDMSO (25 mg/ml)
          Storage -20°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 2 months at -20°C.
          Toxicity Highly Toxic
          Merck USA index14, 10053
          ReferencesCross, M.J., et al. 1995. J. Biol. Chem. 270, 25352.
          Nakamura, I., et al. 1995. FEBS Lett. 361, 79.
          Ferby, I.M., et al. 1994. J. Biol. Chem. 269, 30485.
          Okada, T., et al. 1994. J. Biol. Chem. 269, 3568.
          Wymann, M.P. and Arcaro, A. 1994. Biochem. J. 298, 517.
          Arcaro, A. and Wymann, M.P. 1993. Biochem. J. 296, 297.
          Nakanishi, S., et al. 1992. J. Biol. Chem. 267, 2157.
          Bonser, R.W., et al. 1991. Br. J. Pharmacol. 103, 1237.
          Citation
        • Ying Zhang, Mingjuan Liao and Maria L. Dufau. (2006) Phosphatidylinositol 3-kinase/protein kinase Cγ-induced phosphorylation of Sp1 and p107 repressor release have a critical role in histone deacetylase inhibitor-mediated depression of transcription of the luteinizing hormone receptor gene. Molecular and Cellular Biology 26, 6748-6761.
        • Nadine N. Johnson-Farley, Tatyana Travkina and Daniel S. Cowen. (2005) Cumulative activatino of Akt and consequent inhibition of glycogen synthase kinase-3 by brain-derived neurotrophic factor and insulin-like growth factor-1 in cultured hippocampal neurons. Journal of Pharmacology and Experimental Therapeutics in press,.
        • Xu-Wen Liu, et al. (2005) Tissue inhibitor of metalloproteinase-1 protects human breast epithelial cells from extrinsic cell death: a potential oncogenic activity of tissue inhibitor of metalloproteinase-1. Cancer Research 65, 898-906.
        • Kuntebommanahalli N. Thimmaiah, et al. (2005) Identification of N10-Substituted Phenoxazines as Potent and Specific Inhibitors of Akt Signaling. Journal of Biological Chemistry in press,.
        • Yung-Luen Yu, et al. (2005) MAP kinase-mediated phosphorylation of GATA-1 promotes Bcl-XL expression and cell survival. Journal of Biological Chemistry 280, 29533-29542.
        • Hui Zeng, et al. (2005) Flagellin/TLR5 responses in epithelia reveal intertwined activation of inflammatory and apoptotic pathways. American Journal of Physiology Gastrointestinal and Liver Physiology in press,.