6502 Trypsin, Bovine Pancreas

6502
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      6502-2.5MU
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          Glass bottle 2.5 mu
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          6502-25MU
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              Plastic ampoule 25 mu
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              Description
              OverviewNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides and proteins at the C-terminal side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Note: 1 MU = 1,000,000 units.
              Note: 1 NF unit = 1 USP unit = 3 SU at 30°C, pH 7.6; 1 TAME unit = 19.2 USP units = 57.5 BAEE units; 1 MU = 1,000,000 units.
              Catalogue Number6502
              Brand Family Calbiochem®
              References
              ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
              Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
              Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
              Product Information
              CAS number9002-07-7
              Activity≥2,500 USP units/mg dry weight
              Unit of DefinitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
              EC number3.4.21.4
              FormSolid
              FormulationSalt-free
              Hygroscopic Hygroscopic
              Applications
              Biological Information
              Physicochemical Information
              ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              RTECSYN5075000
              Safety Information
              R PhraseR: 36/37/38-42

              Irritating to eyes, respiratory system and skin.
              May cause sensitization by inhalation.
              S PhraseS: 22-24-26-36/37

              Do not breathe dust.
              Avoid contact with skin.
              In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
              Wear suitable protective clothing and gloves.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Irritant
              Storage -20°C
              Hygroscopic Hygroscopic
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Trypsin, Bovine Pancreas SDS

              Title

              Safety Data Sheet (SDS) 

              Trypsin, Bovine Pancreas Certificates of Analysis

              TitleLot Number
              6502

              References

              Reference overview
              Bergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
              Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
              Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.

              Citations

              Title
            • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.
            • Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision19-May-2008 RFH
              DescriptionNative trypsin from bovine pancreas. Serine protease that specifically hydrolyzes amides, peptides, and proteins at the C-terminus side of arginine and lysine residues. Inhibitors include aprotinin, DFP, and soybean trypsin inhibitor. Has an optimal pH of 8.5-8.8.
              FormSolid
              FormulationSalt-free
              CAS number9002-07-7
              RTECSYN5075000
              EC number3.4.21.4
              ContaminantsChymotrypsin: ≤50 NF units/mg dry weight
              Activity≥2,500 USP units/mg dry weight
              Unit definitionOne USP unit is defined as the amount of enzyme that will digest BAEE and cause an increase in absorbance of 0.003 units at 253 nm per min at 25°C, pH 7.6.
              SolubilityHCl (1 mM), H₂O, or Neutral Buffers
              Storage -20°C
              Hygroscopic
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution in HCl, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Dilute into aqueous buffers (at neutral pH) just prior to use. Following reconstitution in H₂O or buffer, use immediately or aliquot and freeze (-70°C) to minimize autolysis. HCl stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C. H₂O or buffer stock solutions are stable for up to 1 month at -70°C.
              Toxicity Irritant
              Merck USA index14, 9795
              ReferencesBergmeyer, H.U. 1983. Methods of Enzymatic Analysis, 3rd ed. Vol. 3, 17.
              Kostka, V., and Carpenter, F.H. 1964. J. Biol. Chem. 239, 1799.
              Hummel, B.C.W. 1955. Can. J. Biochem. Physiol. 37, 1393.
              Citation
            • Elizabeth A. Lipscomb, et al. (2005) The α6β4 integrin maintains the survival of human breast carcinoma cells in vivo. Cancer Research 65, 10970-10976.