616377 Toxin B, Clostridium difficile - Calbiochem

616377
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      616377-2UG
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          Plastic ampoule 2 μg
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          Description
          OverviewA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42, by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositiol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
          Catalogue Number616377
          Brand Family Calbiochem®
          SynonymsCytotoxin
          References
          ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
          Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
          Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
          Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
          Product Information
          ATP CompetitiveN
          FormLyophilized
          FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
          ReversibleN
          Applications
          Biological Information
          Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
          Primary TargetSmall GTPases, such as Rho, Rac, and Cdc42
          Puritymajor band at >200 kDa
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          RTECSXW5807300
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 36-26

          Wear suitable protective clothing.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Do not freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
          Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Toxin B, Clostridium difficile - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Toxin B, Clostridium difficile - Calbiochem Certificates of Analysis

          TitleLot Number
          616377

          References

          Reference overview
          Mackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
          Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
          Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
          Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-April-2009 JSW
          SynonymsCytotoxin
          DescriptionA high molecular weight glucosyltransferase that inhibits small GTPases, such as Rho, Rac, and Cdc42 by glucosylation of a threonine residue. This results in the shutdown of signal transduction cascades, leading to depolymerization of the cytoskeleton, gene transcription of a number of stress-activated protein kinases (SAPKs), and a decrease in the synthesis of phosphatidylinositol 4,5-bisphosphate. May also block serotonin release, and stimulate the production of phospholipases C and D. Due to its ability to affect depolymerization of the cytoskeleton, toxin B has been shown to inhibit biphasic muscle contraction. Unlike Exoenzyme C3 (Cat. No. 341208), this toxin does not require assistance to permeabilize cells.
          FormLyophilized
          FormulationLyophilized from 50 mM Tris, 50 mM NaCl, 0.1% trehalose, pH 7.5.
          RTECSXW5807300
          Puritymajor band at >200 kDa
          Biological activityCytotoxic as determined in a cell rounding assay using human foreskin cells.
          SolubilitySterile dH₂O. Please see vial label for lot-specific reconstitution volume.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Special InstructionsFollowing reconstitution, refrigerate (4°C). Stock solutions are stable for up to 2 weeks at 4°C. Toxin B activity will decrease after 2 weeks at 4°C following reconstitution.
          Toxicity Irritant
          ReferencesMackay, D.J. and Hall, A. 1998. J. Biol. Chem. 273, 20685.
          Prepens, U., et al. 1996. J. Biol. Chem. 271, 7324.
          Schmidt, M., et al. 1996. Eur. J. Biochem. 240, 707.
          Wilkins, T.D. and Lyerly, D.M. 1996. Trends Microbiol. 4, 49.