69025 T7•Tag® Affinity Purification Kit

69025
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      69025-3
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          Glass bottle 1 kit
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          Description
          OverviewThe T7•Tag® Affinity Purification Kit is designed for rapid immunoaffinity purification of target proteins that carry the T7•Tag sequence (e.g., the 11 amino terminal aa of the T7 gene 10 protein). Purification is based on binding target proteins to T7•Tag Monoclonal Antibody that is covalently coupled to cross-linked agarose beads, washing away unbound proteins, and eluting at pH 2.2. Neutralization Buffer is included to limit protein exposure to low pH. Capacity will vary somewhat between different target proteins, but the beads are standardized to bind a minimum of 300 µg T7•Tag β-galactosidase per milliliter of settled resin. The beads can be used in either batch or column methods and can be regenerated a minimum of five times without loss of binding activity.
          Catalogue Number69025
          Brand Family Novagen®
          References
          Product Information
          Components
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/37/38

          Irritating to eyes, respiratory system and skin.
          S PhraseS: 26-36

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          T7•Tag® Affinity Purification Kit SDS

          Title

          Safety Data Sheet (SDS) 

          T7•Tag® Affinity Purification Kit Certificates of Analysis

          TitleLot Number
          69025

          Citations

          Title
        • Hayakawa, H., et al. 2007. Soluble Journal of Biological Chemistry 282, 26369.
        • Chang-Sik Oh and Steven V. Beer. 2007. Plant Physiology 145, 426.
        • Sukumar, N., et al. 2007. Journal of Bacteriology 189, 3695.
        • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
        • Suzuki, T., et al. 2005. Plant and Cell Physiology 46, 1452.
        • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. 2003. The Cell 113, 127.
        • User Protocols

          Title
          TB055 pET System Manual
          TB125 T7•Tag® Affinity Purification Kit