70044 T7Select® 1-2c DNA

70044
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      Overview

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      70044-3
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          Plastic ampoule 10 μg
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          Description
          OverviewThe T7Select1-2a-c vectors are protein display vectors based on bacteriophage T7. The vector displays an average of 0.1–1 copies of peptides and proteins up to 900 aa in size on the surface of the T7 capsid. Target sequences are fused to the C-terminus of the 10B capsid protein near amino acid 348. T7Select1 phage are grown on a complementing host (BLT5403) that supplies large amounts of the 10A capsid protein from a plasmid. Capsids thus contain mostly 10A protein, along with 0.1–1 copy of 10B fusion protein per virion. The linear genome of T7Select1-2a is 37,245 bp in size. Unique restriction sites are shown on the vector map (TB182).




          This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
          Catalogue Number70044
          Brand Family Novagen®
          References
          Product Information
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          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          T7Select® 1-2c DNA SDS

          Title

          Safety Data Sheet (SDS) 

          T7Select® 1-2c DNA Certificates of Analysis

          TitleLot Number
          70044

          Citations

          Title
        • Stefan Danner and Joel G. Belasco. (2001) T7 phage display: a novel genetic selection system for cloning RNA-binding proteins from cDNA libraries. Procedings of the National Academy of Science 98, 12954-12959.
        • User Protocols

          Title
          TB178 T7Select® System Manual

          Vector Map

          Title
          TB182 T7Select 1-2a-c Vector Map

          Vector Sequence

          Title
          T7Select1-2c Sequence

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          Categories

          Life Science Research > Genomic Analysis > DNA Preparation & Cloning > Cloning > Cloning Vectors
          Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors