03-0161-00 SMC™ Human IL-4 High Sensitivity Immunoassay Kit

03-0161-00
96 well assay  
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityKey Applications
      NhP, R, Fe, Ca, Eq Single Molecule Counting (SMC™)
      Description
      Catalogue Number03-0161-00
      Trade Name
      • SMC™
      DescriptionSMC™ Human IL-4 High Sensitivity Immunoassay Kit
      OverviewThe SMC™ Human IL-4 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
      Background InformationInterleukin 4 (IL-4) is a cytokine produced by activated T cells and has been shown to have effects on both hematopoietic and immunological processes. It can be B cell stimulating and Th2-promoting and is responsible for the immunoglobulin isotype switching to IgG1and IgE. Overproduction of IL-4 is associated with allergies, tumor growth and progression and increased production has even been seen in those infected with HIV.
      References
      Product Information
      Components
      • Assay Buffer
      • IL-4 Coated Beads
      • Standard Diluent
      • IL-4 Detection
      • IL-4 Standard
      • 10X Wash Buffer
      • Buffer D
      • Elution Buffer B
      Precision, %
      • Intra-assay CV% average (n=20): <15%
      • Inter-assay CV% average (n=5): <20%
      Applications
      ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human IL-4 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-4 in human serum and plasma.
      Key Applications
      • Single Molecule Counting (SMC™)
      Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-4 kit, #03-0052-00:
      • Improved options for sample prep and incubation
      • Lyophilization of the standard provides the kit at one storage condition
      • Removal of a plate transfer step
      • Overnight incubation can be used
      • Increased reagent volumes to accommodate automation
      • Validated to run on both SMCxPRO™ and Erenna®
      Biological Information
      Species Reactivity
      • Non-human Primate
      • Rat
      • Feline
      • Canine
      • Horse
      Species Reactivity NoteAlthough IL-4 was detectable in non-human primate, rat, feline, canine, and equine samples, the assay has not been validated for these species.
      Accuracy
      • Minimum of 3 serum samples spiked with average recovery of 94% with a range of 86-101%
      • Minimum of 3 plasma samples spiked with average recovery of 101% with a range of 86-120%
      Physicochemical Information
      Sensitivity
      • Lower Limit of Quantification: 0.04 pg/mL
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size96 well assay
      Transport Information
      Supplemental Information
      Specifications