555460 Rho Inhibitor, Rhosin - Calbiochem

555460
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      Overview

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      555460-25MG
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          Glass bottle 25 mg
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          Description
          OverviewA cell-permeable (quinoxalinylmethylidene-D-Tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber & focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30 µM) in a reversible manner.
          Catalogue Number555460
          Brand Family Calbiochem®
          Synonyms(2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide, G04
          References
          ReferencesShang, X., et al. 2012. Chem. Biol. 19, 699.
          Product Information
          CAS number1173671-63-0
          FormOrange brown powder
          Hill FormulaC₂₀H₁₈N₆O
          Chemical formulaC₂₀H₁₈N₆O
          ReversibleY
          Structure formula ImageStructure formula Image
          Applications
          ApplicationRho Inhibitor, Rhosin, CAS 1173671-63-0, is a cell-permeable compound that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs.
          Biological Information
          Primary TargetRho GTPase
          Primary Target K<sub>i</sub>354 nM for RhoA
          Purity≥93% by HPLC
          Physicochemical Information
          Cell permeableY
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Protect from Light Protect from light
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Rho Inhibitor, Rhosin - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Rho Inhibitor, Rhosin - Calbiochem Certificates of Analysis

          TitleLot Number
          555460

          References

          Reference overview
          Shang, X., et al. 2012. Chem. Biol. 19, 699.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision14-July-2014 JSW
          Synonyms(2R)-2-Amino-3-(1H-indol-3-yl)-Nʹ-((1E)-quinoxalin-6-ylmethylidene)propanehydrazide, G04
          DescriptionA cell-permeable (quinoxalinylmethylidene-D-tryptophanyl)hydrazide that directly targets Rho GEF binding domain (Kd = 354 nM for RhoA), thereby prevents Rho from interacting with its GEFs (including LARG, DBL, LBC, p115 RhoGEF, and PDZ RhoGEF), but not p190RhoGAP, RhoGDI, or its effectors (ROCK, mDia, PKN, Rhoteckin). Shown to suppress mammospheres formation in MCF7, but not the non-tumorigenic MCF10A, cultures and inhibit serum-induced RhoA, RhoB, and RhoC, but not Cdc42 or Rac1, activation, MLC & PAK phosphorylation, as well as stress fiber and focal complex formation in serum-starved NIH3T3 cultures (Effective conc. 30 µM) in a reversible manner. RhoA inactivation by G04 treatment (30 µM) is also demonstrated to promote neurite outgrowth and branching from NGF-treated PC12 cells.
          FormOrange brown powder
          Intert gas (Yes/No) Packaged under inert gas
          CAS number1173671-63-0
          Chemical formulaC₂₀H₁₈N₆O
          Structure formulaStructure formula
          Purity≥93% by HPLC
          SolubilityDMSO (10 mg/ml)
          Storage +2°C to +8°C
          Protect from light
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesShang, X., et al. 2012. Chem. Biol. 19, 699.