553506 RAP, Human, Recombinant, E. coli

553506
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      Overview

      Replacement Information

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      553506-50UG
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          Plastic ampoule 50 μg
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          Description
          OverviewRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
          Catalogue Number553506
          Brand Family Calbiochem®
          SynonymsReceptor Associated Protein
          References
          ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
          Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
          Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
          Bu, G. 2001. Int. Rev. Cytol. 209, 79.
          Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
          Product Information
          FormLiquid
          Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
          Applications
          Biological Information
          Purity≥90% by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          RAP, Human, Recombinant, E. coli SDS

          Title

          Safety Data Sheet (SDS) 

          RAP, Human, Recombinant, E. coli Certificates of Analysis

          TitleLot Number
          553506

          References

          Reference overview
          Li, Y., et al. 2002. Biochemistry 41, 4921.
          Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
          Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
          Bu, G. 2001. Int. Rev. Cytol. 209, 79.
          Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision20-April-2009 JSW
          SynonymsReceptor Associated Protein
          DescriptionRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
          FormLiquid
          Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Purity≥90% by SDS-PAGE
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
          Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
          Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
          Bu, G. 2001. Int. Rev. Cytol. 209, 79.
          Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035