552095 R-Spondin 4, Mouse, Recombinant

552095
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      Overview

      Replacement Information

      Key Specifications Table

      Purity
      ≥95% by SDS-PAGE

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      552095-25UG
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      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
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          Contact Customer Service

          Glass bottle 25 μg
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          Description
          OverviewRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
          Catalogue Number552095
          Brand Family Calbiochem®
          SynonymsCristin 4
          References
          ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
          Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
          Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
          Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
          Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
          Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
          Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
          Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
          Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
          Product Information
          FormSolid
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
          Applications
          Biological Information
          Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
          Purity≥95% by SDS-PAGE
          Physicochemical Information
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          R-Spondin 4, Mouse, Recombinant SDS

          Title

          Safety Data Sheet (SDS) 

          R-Spondin 4, Mouse, Recombinant Certificates of Analysis

          TitleLot Number
          552095

          References

          Reference overview
          Hendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
          Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
          Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
          Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
          Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
          Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
          Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
          Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
          Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision22-August-2011 RFH
          SynonymsCristin 4
          DescriptionRecombinant, mouse R-Spondin 4 consisting of amino acids 19-228 and expressed in Chinese Hamster Ovary cells. Able to induce activation of β-catenin response in a Luciferase assay using HEK293T human embryonic kidney cells. R-spondin 4 is useful as a positive modulator of Wnt/β signaling.
          FormSolid
          FormulationLyophilized from 0.2 µm sterile-filtered PBS, NaCl, EDTA, BSA.
          Purity≥95% by SDS-PAGE
          ContaminantsEndotoxin: <1.0 EU/µg protein
          Biological activityED₅₀ = 5-20 ng/ml in the presence of 10 ng/ml rmWnt-3a, as measured by induction of activation of β-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells.
          SolubilityReconstitute to 200 µg/ml in PBS.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
          Toxicity Standard Handling
          ReferencesHendrickx, M. and L. Leyns. 2008. Develop. Growth Differ. 50, 229.
          Ishii, Y., et al. 2008. J. Invest. Dermatol. 128, 867.
          Kim, K.A., et al. 2008. Mol. Biol. Cell 19, 2588.
          Binnerts, M.E., et al. 2007. Proc. Natl. Acad. Sci. USA 104 14700.
          Nam, J.S., et al. 2007. Gene Expr. Patterns 7, 306.
          Pemberton, T.J., et al. 2007. Dev. Dyn. 236, 2245.
          Bergmann, C., et al. 2006. Am. J. Hum. Genet. 79, 1105.
          Blaydon, D.C., et al. 2006. Nat. Genet. 38, 1245.
          Nam, J.S., et al. 2006. J. Biol. Chem. 281, 13247.