508838 | Puromycin, Dihydrochloride, Sterile-Filtered Aqueous Solution, Cell Culture-Tested - CAS 58-58-2 - Calbiochem

508838
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      Overview

      Replacement Information

      Key Specifications Table

      Empirical FormulaCAS #
      C₂₂H₂₉N₇O₅ • 2HCl 58-58-2

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      5.08838.0001
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          Glass bottle 5 ml
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          Description
          OverviewAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
          Catalogue Number508838
          Brand Family Calbiochem®
          Synonyms(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
          References
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
          Product Information
          CAS number58-58-2
          FormLiquid
          FormulationSupplied at 10 mg/ml in H₂O.
          Hill FormulaC₂₂H₂₉N₇O₅ • 2HCl
          Chemical formulaC₂₂H₂₉N₇O₅ • 2HCl
          ReversibleY
          Applications
          Biological Information
          Primary TargetRNA
          Purity≥98% by HPLC.
          Physicochemical Information
          ContaminantsEndotoxin: ≤10 EU/mg dry weight
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Protect from Light Protect from light
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
          Packaging Information
          Packaged under inert gas Packaged under inert gas
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          References

          Reference overview
          Chow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-May-2014 JSW
          Synonyms(2S)-2-Amino-N-((2S,3S,4R,5R)-5-(6-(dimethylamino)purin-9-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl)-3-(4-methoxyphenyl)
          DescriptionAn aminonucleoside antibiotic that acts as a prokaryotic and eukaryotic protein synthesis inhibitor. Resembles the aminoacyl-adenylyl terminus of aminoacyl-tRNA and competes for binding to the A site of the large ribosomal subunit. Its incorporation into a growing polypeptide results in termination of chain elongation and release of the nascent polypeptidyl-puromycin. Useful for selection of mammalian cell lines expressing a transfected pac gene (Streptomyces alboniger), whose product puromycin acetyltransferase, inactivates the drug via acetylation. Induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells. Recommended range for selection of transfected mammalian cells ranges from 1 to 30 µg/ml.
          FormLiquid
          FormulationSupplied at 10 mg/ml in H₂O.
          Intert gas (Yes/No) Packaged under inert gas
          CAS number58-58-2
          Chemical formulaC₂₂H₂₉N₇O₅ • 2HCl
          Purity≥98% by HPLC.
          ContaminantsEndotoxin: ≤10 EU/mg dry weight
          Storage -20°C
          Protect from light
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C). Aliquots are stable for up to 6 months at -20°C.
          Toxicity Standard Handling
          ReferencesChow, S.C., et al. 1995. Exp. Cell Res. 216, 149.
          Kaufman, S.H., et al. 1993. Cancer Res. 53, 3976.
          de la Luna, S. and Ortin, J. 1992. Methods Enzymol. 216, 376.
          Chow, S.C., et al. 1991. Exp. Cell Res. 216, 149.