71049 Proteinase K Solution, 600 mAU/ml

71049
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      Overview

      Replacement Information

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      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      71049-3
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          Glass bottle 2 ml
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          71049-4
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              Glass bottle 10 ml
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              Description
              OverviewProteinase K is a highly active 28,904-Da serine protease isolated from the fungus Tritirachium album. The enzyme exhibits broad cleavage specificity on native and denatured proteins and is widely used in the purification of DNA and RNA. Its activity is increased in the presence of denaturants such as SDS (1%) and elevated temperature (50-60°C). The recommended working concentration is 50-100 µg/ml for protein removal and enzyme inactivation, and up to 2 mg/ml for tissue treatment. The Proteinase K, Lyophilized powder can be prepared as a 20 mg/ml stock solution in water and stored in aliquots at -20°C. The enzyme is also available as a ready-to-use concentrated stock solution (600 mAU/ml) that is convenient for routine use in most applications. 1 mg of Proteinase K is the equivalent of 30 mAU (AU = Anson unit). The Novagen Proteinase K products are free of detectable DNase and RNase
              Catalogue Number71049
              Brand Family Novagen®
              References
              Product Information
              Unit of DefinitionOne AU (AU = Anson unit) is defined as the amount of enzyme that liberates 1.0 µmol (181 µg) of tyrosine from casein per minute at pH 7.5 at 37°C.
              Applications
              Biological Information
              Physicochemical Information
              ContaminantsFree of detectable DNase and RNase.
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Shipped with Blue Ice or with Dry Ice
              Toxicity Harmful
              Storage -20°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              Proteinase K Solution, 600 mAU/ml SDS

              Title

              Safety Data Sheet (SDS) 

              Proteinase K Solution, 600 mAU/ml Certificates of Analysis

              TitleLot Number
              71049

              Citations

              Title
            • Shannon M. Anderson, et al. (2007) New markers for murine memory B cells that define mutated and unmutated subsets. Journal of Experimental Medicine 204, 2103-2114.
            • Andrew A. Horwitz, et al. (2007) ATP-induced structural transitions in PAN, the proteasome-regulatory ATPase complex in Archaea. Journal of Biological Chemistry 282, 22921-22929.
            • User Protocols

              Title
              TB271 Proteinase K

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              70663 Proteinase K, Lyophilized Show Pricing & Availability

              Categories

              Life Science Research > Genomic Analysis > DNA Preparation & Cloning > DNA Purification > DNA/RNA Clean-Up
              Life Science Research > Proteins and Enzymes > Other Enzymes