539322 Protein G, Peroxidase Conjugate

539322
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539322-500UG
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 500 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewPurified recombinant protein G conjugated to peroxidase. Binds ≥ 99% to human IgG immobilized on a column.
          Catalogue Number539322
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
          Preservative0.02% Thimerosal
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Application NotesDirect Immunoassay (1:5000)
          Application CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          PuritySingle band at 33.8 kDa by SDS-PAGE
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Mercury prohibited To comply with ban of sale of mercury-added products required by The Interstate Mercury Education and Reduction Clearinghouse (IMERC), this product is prohibited to be sold in the following US states: Rhode Island and Connecticut.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Protein G, Peroxidase Conjugate SDS

          Title

          Safety Data Sheet (SDS) 

          Protein G, Peroxidase Conjugate Certificates of Analysis

          TitleLot Number
          539322
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2007 RFH
          ApplicationDirect Immunoassay (1:5000)
          DescriptionPurified recombinant protein G conjugated to peroxidase.
          BackgroundProtein G is a cell-surface protein of Streptococcus species that binds to the Fc portions of immunoglobulins from various species. Like protein A, it is a versatile and economic means to isolate, detect and purify antibodies produced by many animals. Protein G is especially valued because of its ability to react with Human IgG3 and a number of animal immunoglobulins with which protein A does not react well (i.e. bovine, goat, horse, rat and sheep). It also reacts more strongly with mouse immunoglobulins than protein A. Additionally, unlike protein A, it does not react with IgA, IgE and IgM.
          FormLiquid
          FormulationIn 10 mM PBS, 0.5 mM EDTA, 10% mannose, 2% sucrose, 1% BSA, pH 7.6.
          Concentration Label Please refer to vial label for lot-specific concentration
          PuritySingle band at 33.8 kDa by SDS-PAGE
          Preservative0.02% Thimerosal
          CommentsBinds ≥99% to human IgG immobilized on a column. o-Phenylenediamine, 1 mg/ml in citrate buffer, pH 4.5, 0.1% urea hydrogen peroxide at 25°C, is used for testing immunoenzyme reactivity Purity of the recombinant protein G has been determined by presence of a single band at M.W. 33,800 on a 15% SDS-polyacrylamide gel. Specifically binds the Fc region of IgG from various species. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling