16-201 Protein G Agarose/Salmon Sperm DNA, 2.5 mL

2.5 mL  2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
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      Achieve first time ChIP success with EZ-ChIP Assays
      Catalogue Number16-201
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionProtein G Agarose/Salmon Sperm DNA, 2.5 mL
      OverviewRecombinant Protein G covalently bound to agarose by cyanogen bromide linkage. Sonicated salmon sperm DNA blocks non-specific DNA binding sites on protein G agarose when collecting immunocomplexes for chromatin immunoprecipitations (ChIP assays).
      Alternate Names
      • ChIP agarose beads
      • ChIP agaraose G beads
      Background InformationRecombinant protein G has a very high affinity for the Fc regions of IgG molecules. Immoblisation of protein G on beads creates an affinity resin which can be used to purify IgG fractions from crude serum, ascites fluid or cell cuture media.
      Product Information
      Presentation2.5 mL packed beads containing 1 mg sonicated salmon sperm DNA, 2.5 mg BSA and approximately 5 mg recombinant Protein G. Provided as a 50% gel slurry for a final volume of 5 mL per vial. Suspended in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, containing 0.05% sodium azide. Liquid suspension.
      ApplicationRecombinant Protein G covalently bound to agarose beads by cyanogen bromide linkage for use in chromatin immunoprecipitations (ChIP assays). Sonicated salmon sperm DNA is included to block non-specific DNA binding sites on agarose beads. Every lot qualified using an Acetyl Histone H3 ChIP assay.
      Biological Information
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated in Chromatin Immunoprecipitation for use to pre-clear the supernatant from sodium butyrate treated HeLa cells and subsequent immunoprecipitation of DNA cross-linked to acetylated histones
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions1 year at 4°C from date of shipment.
      Packaging Information
      Material Size2.5 mL
      Material Package2.5 mL packed beads provided as a 50% gel slurry for a final volume of 5 mL.
      Transport Information
      Supplemental Information




      Safety Data Sheet (SDS) 

      Certificates of Analysis

      TitleLot Number
      Protein G Agarose/Salmon Sperm DNA2943800
      Protein G Agarose/Salmon Sperm DNA3128120
      Protein G Agarose/Salmon Sperm DNA - 21454572145457
      Protein G Agarose/Salmon Sperm DNA - 23847272384727
      Protein G Agarose/Salmon Sperm DNA - 23971232397123
      Protein G Agarose/Salmon Sperm DNA - 06070359760607035976
      Protein G Agarose/Salmon Sperm DNA - 06090400800609040080
      Protein G Agarose/Salmon Sperm DNA - 07020517870702051787
      Protein G Agarose/Salmon Sperm DNA - 19572681957268
      Protein G Agarose/Salmon Sperm DNA - 20048632004863


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      Molecular microbiology  80  378-90  2011

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      J Cell Sci  123  1716-23  2010

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      J Immunol  183  5518-25  2009

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      Transcriptional regulation of CD4 gene expression by T cell factor-1/beta-catenin pathway.
      Zhaofeng Huang, Huimin Xie, Vassilio Ioannidis, Werner Held, Hans Clevers, Maureen S Sadim, Zuoming Sun
      Journal of immunology (Baltimore, Md. : 1950)  176  4880-7  2006

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