539124 Protease Arrest™ Reagent - Calbiochem

539124
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      539124-1SET
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          Plastic ampoule 1 set
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          Description
          OverviewAn optimized concentration of various reversible and irreversible inhibitors to inhibit serine, cysteine, and calpain proteases. Suitable for the protection of proteins purified from animal tissues, plant tissues, yeast, and bacteria. EDTA is also provided separately to inhibit metalloproteinases. Protease Arrest™ Reagent is provided as a 100X solution that when diluted in extraction buffer at pH 7.0-8.0 inhibits 95-98% of protease activity. Supplied with a data sheet.
          Catalogue Number539124
          Brand Family Calbiochem®
          References
          Product Information
          FormLiquid
          ReversibleN
          Applications
          Biological Information
          Primary TargetSerine, cysteine, and calpain proteases
          Physicochemical Information
          Cell permeableN
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          R PhraseR: 36/38-52/53

          Irritating to eyes and skin.
          Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.
          S PhraseS: 26-61

          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Avoid release to the environment. Refer to special instructions/safety data sheet.
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Special InstructionsFollowing addition to buffers or extracts this product is stable for up to 2 weeks at 4°C or for up to 3 months at -20°C.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          Protease Arrest™ Reagent - Calbiochem SDS

          Title

          Safety Data Sheet (SDS) 

          Protease Arrest™ Reagent - Calbiochem Certificates of Analysis

          TitleLot Number
          539124
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision02-September-2008 RFH
          DescriptionProtease Arrest™ Reagent provides optimized concentration of the various inhibitors, and exhibits excellent inhibitory activity towards various proteases. It is suitable for protection of proteins during purification. It can be used with animal tissues, plant tissues, yeast and bacteria. Protease Arrest™ Reagent contains a blend of both irreversible and reversible inhibitors for serine, and cysteine proteases and for calpains. A vial of EDTA is also provided to inhibit metalloproteases. Please note, however, that some proteins may require divalent cations like Ca2+, Mg2+ or Mn2+ for their activity, as the presence of EDTA may be detrimental to their enzymatic activity. Furthermore, if the protein of interest is purified using immobilized metal chelate affinity chromatography, EDTA must be removed from the buffer before the chromatography. The EDTA solution may be added in the extraction buffer or lysate as needed. Protease Arrest™ Reagent at 1X concentration in extraction buffer at pH 7-8 inhibits over 95% of protease activities. Each set contains the following components

          • Protease-Arrest™ Reagent (100X) 2 ml
          • EDTA (0.5 M) 2 ml
          FormLiquid
          Recommended reaction conditions1. Allow solution to warm to the room temperature. The solution is in suspension form, vortex the vial before removing the solution. 2. Add Protease Arrest™ Reagent 10 µl/ml directly to an appropriate volume of extraction buffer or extract to 1X final concentration. 3. For greater protease inhibition, add Protease Arrest™ Reagent 20-30 µl/ml to get 2-3X final concentration 4. For inhibition of metalloproteases (if the buffer does not contain EDTA), add EDTA (0.5 M) 10 µl/ml directly in an appropriate volume of extraction buffer or extract to 1X final concentration.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing addition to buffers or extracts this product is stable for up to 2 weeks at 4°C or for up to 3 months at -20°C.
          Toxicity Irritant