525200 Phospholipase D, Streptomyces chromofuscus

525200
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      Overview

      Replacement Information

      Key Specifications Table

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      525200-250U
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          Plastic ampoule 250 u
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          525200-U
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              Glass bottle
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              Description
              OverviewNative phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline. Coupled with choline oxidase, it can also be used for the determination of phospholipids in serum.
              Catalogue Number525200
              Brand Family Calbiochem®
              SynonymsPLD
              References
              ReferencesKurz, T., et al. 1993. Circ. Res. 72, 701.
              Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
              Yamamoto, I., et al. 1993. Biochim. Biophys. Acta 1145, 293.
              Product Information
              CAS number9001-87-0
              Activity≥50 units/mg dry weight
              Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
              EC number3.1.4.4
              FormLyophilized brownish solid
              Applications
              Biological Information
              Physicochemical Information
              ContaminantsAatalase, glucose oxidase: none detected
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              R PhraseR: 20/21/22

              Harmful by inhalation, in contact with skin and if swallowed.
              S PhraseS: 36

              Wear suitable protective clothing.
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Ambient Temperature Only
              Toxicity Harmful
              Storage -20°C
              Protect from Moisture Protect from moisture
              Do not freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              SDS

              Title

              Safety Data Sheet (SDS) 

              Certificates of Analysis

              TitleLot Number
              525200

              References

              Reference overview
              Kurz, T., et al. 1993. Circ. Res. 72, 701.
              Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
              Yamamoto, I., et al. 1993. Biochim. Biophys. Acta 1145, 293.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision26-August-2008 RFH
              SynonymsPLD
              DescriptionNative phospholipase D from Streptomyces chromofuscus. Catalyzes the hydrolysis of lecithin to phosphatidic acid and choline; coupled with choline oxidase, it can be used for the determination of phospholipids in serum.
              FormLyophilized brownish solid
              CAS number9001-87-0
              EC number3.1.4.4
              ContaminantsAatalase, glucose oxidase: none detected
              Activity≥50 units/mg dry weight
              Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1 µmol of phosphatidylcholine per min at 37°C, pH 8.0.
              Solubility10 mM Tris-HCl, pH 8.0, 0.05% BSA, and 0.1% (w/v) TRITON® X-100 (1 mg/ml)
              Storage -20°C
              Protect from moisture
              Do Not Freeze Ok to freeze
              Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 6 months at 4°C or for up to 1 year at -20°C.
              Toxicity Harmful
              ReferencesKurz, T., et al. 1993. Circ. Res. 72, 701.
              Natarajan, V., and Garcia, J.G. 1993. J. Lab. Clin. Med. 121, 337.
              Yamamoto, I., et al. 1993. Biochim. Biophys. Acta 1145, 293.