506119 | PhosphoDetect™ Anti-p38 MAP Kinase (pThr¹⁸⁰, pTyr¹⁸²) Rabbit pAb

506119
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ca, H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      506119-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes p38α, β, and γ/ERK6 phosphorylated at Thr180 and Tyr182 in uv-treated HEK293 cells and sorbitol-treated PC12 cells.
          Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number506119
          Brand Family Calbiochem®
          Application Data
          Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
          References
          ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
          Nakagami, H., et al. 2001. Diabetes 50, 1472.
          Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
          Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesImmunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Canine
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          506119

          References

          Reference overview
          Marinissen, M.J., et al. 2001. Genes Dev. 15, 535.
          Nakagami, H., et al. 2001. Diabetes 50, 1472.
          Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
          Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision08-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application Data
          Detection of human p38 MAP kinase phosphorylated at Thr180/Tyr182, by immunoblotting. Sample: Whole cell lysate from HEK293 cells treated with UV irradiation. Primary antibody: PhosphoDetect™ Anti-p38 MAP Kinase (pThr180, pTyr182) Rabbit pAb (Cat. No. 506119) (1:1000) pre-incubated with peptide immunogen (lane 1), a generic phophothreonine-containing peptide (lane 2), a generic phosphotyrosine-containg peptide (lane 3), non-phosphorylated peptide corresponding to the phosphopeptide (lane 4), without peptide (lane 5), with phophopeptide derived from the corresponding region of JNK1&2 (lane 6), and phosphopetide derived from the corresponding region of ERK1&2 (lane 7). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~p38 MAPK phosphorylated at Thr180 and Tyr182.
          Backgroundp38 MAP kinase (MAPK), also called RK and CSBP, is the mammalian homologue of yeast HOG kinase and participates in a cascade that controls cellular responses to cytokines and stress. Like the SAPK/JNK pathway, p38 MAPK is activated by a variety of cellular stresses including osmotic shock, inflammatory cytokines, lipopolysaccharides (LPS), UV light, and growth factors. Both MKK3 and SEK phosphorylate p38 on tyrosine and threonine resulting in p38 activation. Activated p38 MAPK has been shown to phosphorylate and activate MAPKAP kinase-2 and to phosphorylate the transcription factors ATF-2 and Max.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr¹⁸⁰ and Tyr¹⁸² phosphorylation sites of human p38 MAP Kinase
          IsotypeIgG
          Speciescanine, human, mouse, rat
          Positive controlUV irradiated HEK293 cells or sorbitol-treated PC12 cells
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsDoes not appreciably recognize the corresponding phosphorylated forms of either p42/44 MAPK or SAPK/JNK MAPK. The immunogen sequence is 100% conserved in p38α, β, and γ. The immunogen sequence is also 100% conserved in mouse, moneky, and carp p38, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. When diluted 1:1000, there is sufficient antibody for 10 immunoblots at 10 ml per blot. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesMarinissen, M.J., et al. 2001. Genes Dev. 15, 535.
          Nakagami, H., et al. 2001. Diabetes 50, 1472.
          Wilsbacher, J.L., et al. 1999. J. Biol. Chem. 274, 16988.
          Raingeaud, J., et al. 1995. J. Biol. Chem. 270, 7420.
          Zervos, A.S., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 10531.
          Han, J., et al. 1994. Science 265, 808.
          Lee, J.C., et al. 1994. Nature 372, 739.
          Rouse, J., et al. 1994. Cell 78, 1027.

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