553009 PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb

553009
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service
      View Pricing & Availability

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      553009-10T
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          Plastic ampoule 10 t
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes c-Raf phosphorylated at Tyr340 and Tyr341 in HEK293 cells overexpressing c-Raf.
          Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number553009
          Brand Family Calbiochem®
          Application Data
          Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
          Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
          Mason, C.S. et al. 1999. EMBO J. 18, 2137.
          Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
          Moelling, K. et al. 1984. Nature 312, 558.
          Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
          Product Information
          FormLiquid
          FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
          Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunocytochemistry
          Application NotesImmunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-c-Raf (pTyr³⁴⁰, Tyr³⁴¹) Rabbit pAb Certificates of Analysis

          TitleLot Number
          553009

          References

          Reference overview
          Yan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
          Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
          Mason, C.S. et al. 1999. EMBO J. 18, 2137.
          Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
          Moelling, K. et al. 1984. Nature 312, 558.
          Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-August-2007 RFH
          ApplicationImmunoblotting (1:1000)
          Immunocytochemistry (see comments)
          Application Data
          Detection of human c-Raf, phosphorylated on Tyr340, by immunoblotting. Samples: Whole cell lysate from HEK293 cells overexpressing c-Raf left unstimulated (lane 1) or stimulated with active Src (lane 2-5). Primary antibody: PhosphoDetect™ Anti-c-Raf (pTyr340, Tyr341) Rabbit pAb (Cat. No. 553009) (1:000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~74 KDa c-Raf protein phosphorylated on Tyr340/341.
          BackgroundThe raf family of proto-oncogenes consists of three members: A-raf-1, B-raf, and c-raf-1 which show the greatest degree of homology in the carboxy-terminal half of the molecule. In the case of c-raf-1 this region has been shown to have protein (serine/threonine) kinase activity. C-raf was first identified in an oncogeneic form, v-raf, which is the oncogene of the transforming virus MSV. The raf-1 gene is the most studied of the three and encodes a protein with an apparent molecular mass of ~74 kDa. Raf is widely expressed and plays an important role in cell proliferation and differentiation. Raf relays signals related to the activation of growth factor receptor to the activation of MAPK and utlimately to the nucleus. Activation of raf itself is dependent on tyrosine (341) phosphorylation. Tyrosine phosphorylation is accomplished by Src tyrosine kinase.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁴⁰ and Tyr³⁴¹, phosphorylation sites of human c-Raf (Raf-1)
          IsotypeIgG
          Specieshuman, mouse, rat
          Positive controlHEK293 cells over expressing c-Raf, phosphorylated in vitro by Src.
          FormLiquid
          FormulationIn phosphate buffer (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (+/- 0.1).
          Preservative≤0.1% sodium azide
          CommentsThe immunogen sequence is 100% conserved in mouse and rat, but cross-reactivity has not been tested. This antibody has also been reported to work for immunocytochemistry. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesYan, F., and Polk, D.B. 2001. Cancer Res. 61, 963.
          Yip-Schneider, M.T., et al. 2000. Biochem. J. 351, 151.
          Mason, C.S. et al. 1999. EMBO J. 18, 2137.
          Bonner, T.I. et al. 1985. Mol. Cell. Biol. 5, 1400.
          Moelling, K. et al. 1984. Nature 312, 558.
          Rapp, U.R. et al. 1983. Proc. Natl. Acad. Sci. USA 80, 4218.

          Related Products & Applications

          Suggested Product

          Catalog Number Description  
          71296 PhosphoSafe™ Extraction Reagent Show Pricing & Availability

          Related Products By: Brand Facete

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies