PS1013 PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb

PS1013
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PS1013-10T
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          Plastic ampoule 10 t
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          Description
          OverviewRecognizes the ~200 kDa VEGFR-2 protein phosphorylated at Tyr1054/1059 in porcine pancreatic cells transfected with a CSF-1/mouse VEGFR-2 chimeric receptor.
          Catalogue NumberPS1013
          Brand Family Calbiochem®
          SynonymsAnti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
          Application Data
          Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
          References
          ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
          Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
          Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
          Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
          Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Application NotesImmunoblotting (1:1000)
          Application CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Mouse
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Irritant
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PS1013

          References

          Reference overview
          Meyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
          Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
          Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
          Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision15-August-2007 RFH
          SynonymsAnti-KDR/Flk-1, Phospho-Specific (Tyr1054/1059), Anti-VEGF Receptor 2, Phospho-specific (pTyr1054/1059)
          ApplicationImmunoblotting (1:1000)
          Application Data
          Detection of mouse VEGFR2, phosphorylated at Tyr1054/1059, by immunoblotting. Samples: Whole cell lysate from porcine aortic endothelial (PAE) cells transfected with CSF-1/mouse VEGFR2 chimeric receptor left unstimulated (lanes 1,2) or stimulated with CSF-1 (lanes 3-8). Primary antibodies: PhosphoDetect™ Anti-VEGFR2 (pTyr1054/1059) Rabbit pAb (Cat. No. PS1013) (0.5 µg/ml) pre-incubated without peptide (lanes 2,4,5), with non-phosphopeptide corresponding to the immunogen (lane 6), a generic phosphotyrosine-containing peptide (lane 7), or the phosphopeptide immunogen (lane 8) or pan anti-VEGFR2 antibody (lanes 1,3). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~200 kDa VEGFR2 protein phosphorylated at Tyr1054/1059.
          BackgroundVascular Endothelial Growth Factor Receptor 2 (VEGFR2) is receptor tyrosine kinase that serves as a key mediator in angiogenesis and plays an essential role in embryonic development, wound healing, cell proliferation, migration, and differentiation. It contains seven immunoglobulin-like sequences in the extracellular domain and exhibits sequence similarity to Flt-1 (FMS-like tyrosine kinase 1) and Flt-4 (FMS-like tyrosine kinase 4). Upon binding to its ligand, VEGF, VEGFR2 undergoes ligand-induced dimerization, which activates its intrinsic tyrosine kinase activity resulting in auto- and trans-phosphorylation on specific tyrosine residues in the cytoplasmic domain. These tyrosine residues, when phosphorylated, are involved as docking sites to recruit molecules containing Src homology 2 (SH2) or phosphotyrosine-binding domains and to convey signals to downstream effectors. Phosphorylation of VEGFR2 at Tyr1054 and Tyr1059 within the activation loop is required for required for activation of VEGFR2 and enhances its intrinsic tyrosine kinase activity. Phosphorylation at Tyr1059 is also responsible for VEGF-induced intracellular Ca2+ mobilization.
          HostRabbit
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr1054/1059 phosphorylation sites of mouse and human VEGFR-2
          IsotypeIgG
          Speciesmouse
          Positive controlPorcine aortic endothelial (PAE) cells transfected with a CSF-1/mouse VEGFR2 chimeric receptor
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg2+ and Ca2+), 1 mg/ml BSA, 50% glycerol, pH 7.3 (± 0.1).
          Preservative≤0.1% sodium azide
          CommentsThe immunogen sequence is 100% conserved in human and rat VEGFR2, but reactivity has not been tested. The corresponding phosphorylation site in VEGFR3 is 100% conserved, but cross-reactivity has not been tested. When used at a 1:1000 dilution there is sufficient antibody for 10 immunoblots at 10 ml/blot. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Irritant
          ReferencesMeyer, R.D., et al. 2002. J. Biol. Chem. 277, 27081.
          Zeng, H., et al. 2001. J. Biol. Chem. 276, 32714.
          Sato, Y., et al. 2000. Ann. N.Y. Acad. Sci. 902, 201.
          Rahimi, N., et al. 1999. Mol. Biol. Cell 10, 3401.