525320 PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20) Peroxidase Conjugate

525320
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      525320-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewPeroxidase conjugate of Anti-Phosphotyrosine Mouse mAb (PY20), Cat. No. 525295. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
          Catalogue Number525320
          Brand Family Calbiochem®
          References
          ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
          Product Information
          FormLiquid
          FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
          Preservative0.1% Proclin
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesELISA (1:2000)
          Immunoblotting (1:500-1:2000)
          Immunohistochemistry (1:500)
          Application CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
          ClonePY20
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          525320

          References

          Reference overview
          Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-December-2007 JSW
          ApplicationELISA (1:2000)
          Immunoblotting (1:500-1:2000)
          Immunohistochemistry (1:500)
          DescriptionPurified mouse monoclonal antibody conjugated to horseradish peroxidase. Recognizes a broad spectrum of tyrosine-phosphorylated proteins.
          BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
          HostMouse
          ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
          ClonePY20
          IsotypeIgG2b
          Speciesa broad range of species
          FormLiquid
          FormulationIn PBS, 50% glycerol, 1% BSA, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative0.1% Proclin
          CommentsSpecific for tyrosine phosphorylated proteins in both the native and denatured states. Binding is inhibited by phosphotyrosine; does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosine containing proteins and will adsorb the antibody. Use a buffer with < 150 mM NaCl and divalent cations not much >1 mM because this antibody's binding is inhibited by high salt concentrations and divalent cations. Variables associated with assay conditions will dictate the proper working dilution.
          Storage +2°C to +8°C
          Do Not Freeze Ok to freeze
          Toxicity Standard Handling
          ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.