525295 | PhosphoDetect™ Anti-Phosphotyrosine Mouse mAb (PY20)

525295
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      A Broad Range Of Species M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      525295-100UG
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          Plastic ampoule 100 μg
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          Description
          OverviewRecognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states in crude extracts.
          Catalogue Number525295
          Brand Family Calbiochem®
          References
          ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
          Product Information
          FormLiquid
          FormulationIn 10 mM sodium phosphate, pH 7.4.
          Positive controlAny cell lysate or tissue extract
          Preservative≤0.1% sodium azide
          Applications
          Application ReferencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.
          Key Applications Immunocytochemistry
          Immunoprecipitation
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (see comments)
          Immunoblotting (1:500-1:1000, see application references)
          Frozen Sections (see application references)
          Immunocytochemistry (see application references)
          Immunoprecipitation (see comments)
          Application CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
          ClonePY20
          HostMouse
          IsotypeIgG2b
          Species Reactivity
          • A Broad Range Of Species
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          525295

          References

          Reference overview
          Glenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision03-December-2007 JSW
          ApplicationELISA (see comments)
          Immunoblotting (1:500-1:1000, see application references)
          Frozen Sections (see application references)
          Immunocytochemistry (see application references)
          Immunoprecipitation (see comments)
          DescriptionPurified mouse monoclonal antibody. Recognizes a broad spectrum of tyrosine-phosphorylated proteins in their native and denatured states.
          BackgroundCellular activation, proliferation and differentiation are signaled by phosphorylation of tyrosine residues. Growth factor receptors and oncogene protein kinases are associated with tyrosine phosphorylation. Anti-phosphotyrosine antibodies are indispensable for the study of cellular tyrosine phosphorylation.
          HostMouse
          ImmunogenKLH conjugated to phosphotyrosine, glycine, and alanine
          ClonePY20
          IsotypeIgG2b
          Speciesa broad range of species
          Positive controlAny cell lysate or tissue extract
          FormLiquid
          FormulationIn 10 mM sodium phosphate, pH 7.4.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsBinding is inhibited by phosphotyrosine. Does not react with threonine or serine phosphorylated residues on proteins. Do not use milk as a blocking agent with this antibody. Milk contains many phosphotyrosines and will adsorb the antibody. This antibody has also been reported to work for ELISA and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesGlenney, J.R., et al. 1988. J. Immunol. Methods 109, 277.
          Application referencesImmunoblotting Dixon, B.S., et al. 1996. J. Biol. Chem. 271, 13324. Frozen Sections, Immunocytochemistry Bao, Z.Z., et al. 1993. J. Cell Sci. 106, 579.