527460 | PhosphoDetect™ Anti-PKR (pThr⁴⁵¹) Rabbit pAb

527460
Price could not be retrieved
Minimum Quantity needs to be mulitiple of
Upon Order Completion More Information
You Saved ()
 
Request Pricing
Limited AvailabilityLimited Availability
Stocked 
Discontinued
Limited Quantities Available
Available
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      View Pricing & Availability
      Click To Print This Page

      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M Rb Polyclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      527460-10T
      Retrieving availability...
      Limited AvailabilityLimited Availability
      Stocked 
      Discontinued
      Limited Quantities Available
      Available
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service

          Plastic ampoule 10 t
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity needs to be mulitiple of
          Upon Order Completion More Information
          You Saved ()
           
          Request Pricing
          Description
          OverviewRecognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
          Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
          Catalogue Number527460
          Brand Family Calbiochem®
          SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
          Application Data
          Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          References
          ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
          Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
          Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
          Product Information
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
          Positive controlIFNγ-stimulated HeLa cells.
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunoblotting (Western Blotting)
          Immunohistochemistry
          Application NotesImmunoblotting (1:1000)
          Immunohistochemistry (see comments)
          Application CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          • Mouse
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage ≤ -70°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          527460

          References

          Reference overview
          Cuddihy, A.R., et al.1999. Oncogene 8, 2690.
          Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
          Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision13-August-2007 RFH
          SynonymsAnti-Double-stranded RNA-dependent Protein Kinase, Phospho-Specific (Thr⁴⁵¹)
          ApplicationImmunoblotting (1:1000)
          Immunohistochemistry (see comments)
          Application Data
          Detection of human PKR, phosphorylated on Thr 451, by immunoblotting. Samples: Whole cell lysates from HeLa cells left unstimulated (lane 1) or stimulated with IFNγ and calyculin (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-PKR (pThr 451) Rabbit pAb (Cat. No. 527460) (1:000) pre-incubated without peptide (lanes 1-2), non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphotyrosine-containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
          DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phophorylation site, followed by immunoaffinity chromatography. Recognizes the ~65-68 kDa PKR protein phosphorylated at Thr451.
          BackgroundPKR is an interferon-inducible serine/threonine kinase activated by double stranded RNA. The activation of PKR is accompanied by phosphorylation at multiple sites, including Thr446 and Thr451 in the PKR activation loop. PKR phosphorylates the α-subunit of the eukaryotic initiation factor-2 and inhibits protein synthesis. PKR also phosphorylates p53 and plays a role in the interferon response to viral infections.
          HostRabbit
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr⁴⁵¹ phosphorylation site of human PKR (double-stranded RNA-dependent protein kinase)
          IsotypeIgG
          Specieshuman, mouse
          Positive controlIFNγ-stimulated HeLa cells.
          FormLiquid
          FormulationIn Dulbecco's PBS (without Mg+2 or Ca+2), 0.1% BSA, 50% glycerol, pH 7.3.
          Preservative≤0.1% sodium azide
          CommentsWhen used at a 1:1000 dilution there is sufficient antibody for 10 blots at 10 ml/blot. This antibody has also been reported to work for immunohistochemistry. Variables associated with assay conditions will dictate the optimal dilution.
          Storage ≤ -70°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
          Toxicity Standard Handling
          ReferencesCuddihy, A.R., et al.1999. Oncogene 8, 2690.
          Srivastava, S.P., et al. 1998. J. Biol. Chem. 273, 2416.
          Clemens, M.J. 1997. Int. J. Biochem. Cell Biol. 29, 945.