AP1062 PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb

AP1062
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, Pm, R Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      AP1062-50UL
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          Plastic ampoule 50 ul
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          AP1062-5MG
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              Plastic ampoule 5 mg
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              Description
              OverviewRecognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293 in HEK293 cells.
              Catalogue NumberAP1062
              Brand Family Calbiochem®
              SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
              Application Data
              Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

              Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

              Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
              References
              ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
              Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
              Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
              Product Information
              FormLiquid
              FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
              Positive controlHEK293 cells
              Preservative≤0.1% sodium azide
              Applications
              Key Applications Immunoblotting (Western Blotting)
              Immunocytochemistry
              Immunoprecipitation
              Application NotesImmunoblotting (1:10,000)
              Immunoprecipitation (1:50)
              Immunocytochemistry (1:500)
              Application CommentsAntibody should be titrated for optimal results in individual systems.
              Biological Information
              Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
              ImmunogenHuman
              HostRabbit
              IsotypeIgG
              Species Reactivity
              • Human
              • Mouse
              • Primate
              • Rat
              Antibody TypePolyclonal Antibody
              Physicochemical Information
              Dimensions
              Materials Information
              Toxicological Information
              Safety Information according to GHS
              Safety Information
              Product Usage Statements
              Storage and Shipping Information
              Ship Code Blue Ice Only
              Toxicity Standard Handling
              Storage -20°C
              Do not freeze Ok to freeze
              Packaging Information
              Transport Information
              Supplemental Information
              Specifications

              Documentation

              PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb SDS

              Title

              Safety Data Sheet (SDS) 

              PhosphoDetect™ Anti-PDH-E1α (pSer²⁹³) Rabbit pAb Certificates of Analysis

              TitleLot Number
              AP1062

              References

              Reference overview
              Seifert, F., et al. 2007. Biochemistry 21, 6277.
              Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
              Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.

              Citations

              Title
              Madhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.
              Data Sheet

              Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

              Revision30-August-2011 JSW
              SynonymsAnti-Pyruvate Dehydogenase pSer²⁹³ Rabbit pAb
              ApplicationImmunoblotting (1:10,000)
              Immunoprecipitation (1:50)
              Immunocytochemistry (1:500)
              Application Data
              Detection of human PDH-E3α, phosphorylated at Ser293, by immunocytochemistry. Samples: HEK293 cells treated with DCA (bottom panels) or left untreated (top panels) and subsequently fixed with 4% paraformaldehyde. Primary antibody: PhosphoDetect™ Anti-PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:500). Detection: fluorescence (red) using DAPI to counterstain the nuclei (blue) and AlexaFluor®-Phalloidin to counterstain actin filaments (green).

              Detection of human PDH-E1α, phosphorylated at Ser293, by immunoprecipitation, followed by immunoblotting. Samples: Whole cell lysate (375 µg) from HEK293 cells. Antibody for IP: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:50). . Antibodyfor IB: Anti-PDH-E1α (pSer293 Rabbit pAb (Cat. No. AP1062) (1:1000) Detection: chemiluminescence.

              Detection of human PDH-E1α, phosphorylated at Ser293, by immunoblotting. Samples: Whole cell lysate from HEK293 cells left untreated (lane 1) and HEK293 cells treated with dichloroacetate. Primary antibody: PhosphoDetect™ Anti PDH-E1α (pSer293) Rabbit pAb (Cat. No. AP1062) (1:10,000). Detection: chemiluminescence.
              DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~44 kDa PDH-E1α protein phosphorylated at Ser293.
              BackgroundPyruvate dehydogenase (PDH) complex (PDC) catalyzes the irreversible conversion of pyruvate to acetyl CoA, providing the link between glycolyis and the TCA cycle. Dysregulation of the PDC in skeletal muscle has been implicated in type 2 diabetes and mutations in the PDC result in various mitochondrial diseases. The PDC is a multienzyme complex composed of several copies of three catalytic proteins (E1, E2, and E3): one structural (E3-binding protein) and two regulatory proteins (PDH kinase [PDK] and PDH phosphatase [PDP]). The activity of the PDC complex is mainly regulated by phosphorylation-dehosphorylation of three specific serine residues (232, 293, 300) on the E1 subunit. Phosphorylation of PDH determines the activity of the complex. Phosphorylation by one of four PDK isoforms inactivates the complex, while removal of the phosphate by one of two PDP isoforms activates the complex. The activity of the PDP1 isoform is sensitive to Ca2+, while the PDK isoforms are regulated by the reduction and acetylation status of the E2 subunit, pyruvate, and the small molecule the anti cancer dichloroacetate. (DCA).
              HostRabbit
              Immunogen speciesHuman
              Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser 293 phosphorylation site of human PDH-E1α
              IsotypeIgG
              Specieshuman, mouse, primate, rat
              Positive controlHEK293 cells
              FormLiquid
              FormulationIn 150 mM NaCl, 100 mM Tris-Glycine, pH 7.4.
              Preservative≤0.1% sodium azide
              CommentsAntibody should be titrated for optimal results in individual systems.
              Storage -20°C
              Do Not Freeze Ok to freeze
              Toxicity Standard Handling
              ReferencesSeifert, F., et al. 2007. Biochemistry 21, 6277.
              Lee, J., et al. 2007. Mol. Cell Prot. 4, 669.
              Korotchkina, L.G., et al. 2001. J. Biol. Chem. 40, 37223.
              CitationMadhok, B.M., et al. 2010. British Journal of Cancer 102, 1746.