NE1022 PhosphoDetect™ Anti-Neurofilament H Mouse mAb (SMI-31)

NE1022
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      Ch, Ma, Xn M Monoclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      NE1022-100UL
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          Glass bottle 100 ul
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          Description
          OverviewRecognizes the ~180-200 kDa phosphorylated neurofilament H protein in rat central nervous system (CNS) cytoskeletal preparations. Also weakly recognizes phosphorylated NF-M protein.
          Catalogue NumberNE1022
          Brand Family Calbiochem®
          Application Data
          Detection of phosphorylated rat neurofilament H by staining frozen sections. Sample: Rat brain. Primary antibody: PhosphoDetect™ Anti-Neurofilament-H Mouse mAb (SMI-31) (Cat. No. NE1022) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
          References
          ReferencesRaina, A.K., et al. 1999. Neuroreport 10, 1355.
          Yang, C.C., et al. 1998. Brain 121, 1089.
          Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem. 271, 30404.
          Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol. 55, 774.
          Xiao, J. and Monteiro, M.J. 1994. J. Neurosci. 14, 1820.
          Product Information
          FormLiquid
          FormulationIn PBS and Thimerosal
          Positive controlRat brain or central nervous system cytoskeletal preparations
          Preservative≤0.1% sodium azide
          Applications
          Key Applications Immunocytochemistry
          Paraffin Sections
          Enzyme-Linked Immunosorbent Assay
          Frozen Sections
          Immunoblotting (Western Blotting)
          Application NotesELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000, see comments)
          Paraffin Sections (1:1000, heat pre-treatment required, see comments)
          Immunoprecipitation (see comments)
          Application CommentsStrongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin's fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenhomogenized, hypothalami from Fischer 344 rat brain
          ImmunogenRat
          CloneSMI-31
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Chicken
          • Mammals
          • Xenopus
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-Neurofilament H Mouse mAb (SMI-31) SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-Neurofilament H Mouse mAb (SMI-31) Certificates of Analysis

          TitleLot Number
          NE1022

          References

          Reference overview
          Raina, A.K., et al. 1999. Neuroreport 10, 1355.
          Yang, C.C., et al. 1998. Brain 121, 1089.
          Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem. 271, 30404.
          Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol. 55, 774.
          Xiao, J. and Monteiro, M.J. 1994. J. Neurosci. 14, 1820.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision11-October-2016 JSW
          ApplicationELISA (1:1000)
          Frozen Sections (1:1000, see comments)
          Immunoblotting (1:1000)
          Immunocytochemistry (1:1000, see comments)
          Paraffin Sections (1:1000, heat pre-treatment required, see comments)
          Immunoprecipitation (see comments)
          Application Data
          Detection of phosphorylated rat neurofilament H by staining frozen sections. Sample: Rat brain. Primary antibody: PhosphoDetect™ Anti-Neurofilament-H Mouse mAb (SMI-31) (Cat. No. NE1022) (1:1000). Detection: fluorescence (green) with Hoechst 33342 counterstain.
          DescriptionMouse monoclonal antibody supplied as purified antibody. Recognizes the ~180-200 kDa phosphorylated neurofilament H protein.
          BackgroundNeurofilaments are a type of intermediate filament that serve as major constituent of the cytoskeleton of axons. They are the most abundant fibrillar components of the axon and are composed of three intertwined protofibrils. The neurofilament triplet proteins (NF-L, 68/70 kDa; NF-M, 160 kDa; and NF-H, 200 kDa) are found in both the central and peripheral nervous system and are usually neuron-specific. NF-H and NF-M both require the presence of NF-L protein to co-assemble. NF-H and NF-M become highly phosphorylated after newly formed neurofilaments enter the axon. Neurofilament staining is observed in neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas, and have also been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung are also known to express neurofilaments.
          HostMouse
          Immunogen speciesRat
          Immunogenhomogenized, hypothalami from Fischer 344 rat brain
          CloneSMI-31
          IsotypeIgG₁
          SpeciesXenopus, chicken, mammalian
          Positive controlRat brain or central nervous system cytoskeletal preparations
          FormLiquid
          FormulationIn PBS and Thimerosal
          Preservative≤0.1% sodium azide
          CommentsStrongly recognizes phosphorylated neurofilament H and, to a lesser extent, phosphorylated neurofilament M. By immunocytochemistry this antibody broadly stains thick and thin axons and some dendrites, such as basket cell dendrites, but not Purkinje cell dendrites. Does not generally stain nerve cell bodies or other cells and tissues except peripheral axons. May also stain neuronal cell bodies in pathological conditions. Aberrant phosphorylation of neurofilament H in cell bodies can be demonstrated in neuronal cell cultures following treatment with agents that induce stress-activated protein kinase. This antibody is reported to co-immunoprecipitate neurofilament-associated kinase (NAK 115) via interaction of the antibody with the tail domain of neurofilament H. Phosphatase treatment of tissue sections or immunoblotting samples abolishes antibody reactivity. Reactivity is unaffected by trypsin treatment of samples. Tissues and cultured cells can be fixed with a variety of paraformaldehyde- or formaldehyde-containing fixatives, including Bouin's fixative. Post-fixation in cold methanol or methanol/hydrogen peroxide facilitates access of the antibody to the epitope in frozen sections or thick tissue sections fixed in 4% paraformaldehyde and in cultured cells. For staining formalin-fixed, paraffin sections it is recommended that the de-paraffinized tissue be autoclaved in dH2O for 10 min or boiled in Tris-buffered saline, pH 9.0, in a microwave for 15 min to expose the epitope. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesRaina, A.K., et al. 1999. Neuroreport 10, 1355.
          Yang, C.C., et al. 1998. Brain 121, 1089.
          Giasson, B.I and Mushynski, W.E. 1996. J. Biol. Chem. 271, 30404.
          Mirabella, M., et al. 1996. J. Neuropath. Exp. Neurol. 55, 774.
          Xiao, J. and Monteiro, M.J. 1994. J. Neurosci. 14, 1820.