PC635 PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb

PC635
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H Rb Polyclonal Antibody

      Pricing & Availability

      Catalog Number AvailabilityPackaging Qty/Pack Price Quantity
      PC635-50UL
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          Plastic ampoule 50 ul
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          Description
          OverviewRecognizes the ~95 kDa nibrin protein phosphorylated at Ser343 in δ-irradiated lymphoblastoid cells.
          Catalogue NumberPC635
          Brand Family Calbiochem®
          SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
          References
          ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
          Lim, D.S., et al. 2000. Nature 404, 613.
          Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
          Zhong, Q., et al. 1999. Science 285, 747.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
          Varon, R., et al. 1998. Cell 93, 467.
          Product Information
          FormLiquid
          FormulationSerum diluted in PBS.
          Positive controlγ-irradiated lymphoblastoid cells
          PreservativeNone
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (1:16,000)
          Immunoblotting (1:1000)
          Immunoprecipitation (1 µl/500 µg cell extract)
          Application CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
          ImmunogenHuman
          HostRabbit
          IsotypeIgG
          Species Reactivity
          • Human
          Antibody TypePolyclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Standard Handling
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb SDS

          Title

          Safety Data Sheet (SDS) 

          PhosphoDetect™ Anti-NBS1 (pSer³⁴³) Rabbit pAb Certificates of Analysis

          TitleLot Number
          PC635

          References

          Reference overview
          Gatei, M., et al. 2000. Nat. Genet. 25, 115.
          Lim, D.S., et al. 2000. Nature 404, 613.
          Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
          Zhong, Q., et al. 1999. Science 285, 747.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
          Varon, R., et al. 1998. Cell 93, 467.
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision12-October-2007 RFH
          SynonymsAnti-Nibrin (pSer³⁴³) Rabbit pAb
          ApplicationELISA (1:16,000)
          Immunoblotting (1:1000)
          Immunoprecipitation (1 µl/500 µg cell extract)
          DescriptionRabbit polyclonal antibody supplied as adsorbed diluted serum. Recognizes the ~95 kDa nibrin (NBS1) protein phosphorylated at Ser343.
          BackgroundNibrin, a ~95 kDa double stranded DNA repair protein, is mutated in Nijmegen breakage syndrome (NBS). Patients with NBS exhibit chromosomal instability, resulting in hypersensitivity to irradiation and cancer predisposition. Nibrin forms a manganese-sensitive complex with hMre11 and hRAD50 that has single strand DNA endonuclease and exonuclease activities and is likely to be important for DNA excision, repair, and stability. This nibrin/Mre11/RAD50 complex is inhibited by BRCA1. The ATM (ataxia telangiectasia mutated) kinase has been shown to phosphorylate nibrin in an S-phase checkpoint pathway. Nibrin levels are elevated in testis tissue, suggesting a possible role in meiotic recombination. Nibrin may also be involved in telomere length maintenance.
          HostRabbit
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide [TPGPSLpSQGVSVD(C)] corresponding to amino acids 337-349 surrounding the Ser³⁴³ phosphorylation site of human nibrin, conjugated to diphtheria toxoid
          IsotypeIgG
          Specieshuman
          Positive controlγ-irradiated lymphoblastoid cells
          FormLiquid
          FormulationSerum diluted in PBS.
          PreservativeNone
          CommentsThis antibody preparation has been adsorbed against non-phosphopeptide that corresponds to the immunogen sequence. Does not detect non-phosphorylated nibrin protein. Antibody should be titrated for optimal results in individual systems.
          Storage -20°C
          Avoid freeze/thaw
          Do Not Freeze Ok to freeze
          Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
          Toxicity Standard Handling
          ReferencesGatei, M., et al. 2000. Nat. Genet. 25, 115.
          Lim, D.S., et al. 2000. Nature 404, 613.
          Wu, G., et al. 2000. J. Biol. Chem. 275, 30618.
          Zhong, Q., et al. 1999. Science 285, 747.
          Carney, J.P., et al. 1998. Cell 93, 477.
          Trujillo, K.M., et al. 1998. J. Biol. Chem. 273, 21447.
          Varon, R., et al. 1998. Cell 93, 467.