442706 | PhosphoDetect™ Anti-MAP Kinase ERK1/ERK2 (pThr²⁰²/Tyr²⁰⁴) Mouse mAb (12D4)

442706
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      Overview

      Replacement Information

      Key Specifications Table

      Species ReactivityHostAntibody Type
      H, M, R M Monoclonal Antibody

      Pricing & Availability

      Catalog NumberAvailability Packaging Qty/Pack Price Quantity
      442706-1SET
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          Description
          OverviewRecognizes the ~42-44 kDa ERK1 and ERK2 proteins phosphorylated at Thr202 and Tyr204 in vanadate-treated A431 cells. Supplied with a control lysate.
          Catalogue Number442706
          Brand Family Calbiochem®
          SynonymsAnti-MAPK1/2, Activated
          References
          Product Information
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
          Positive controlvanadate-treated A431 cells (supplied)
          Preservative≤0.1% sodium azide (antibody only)
          Applications
          Key Applications Enzyme-Linked Immunosorbent Assay
          Immunoblotting (Western Blotting)
          Immunoprecipitation
          Application NotesELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
          Application CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Biological Information
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
          ImmunogenHuman
          Clone12D4
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          • Mouse
          • Rat
          Antibody TypeMonoclonal Antibody
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Ambient Temperature Only
          Toxicity Standard Handling
          Storage -20°C
          Do not freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications

          Documentation

          SDS

          Title

          Safety Data Sheet (SDS) 

          Certificates of Analysis

          TitleLot Number
          442706
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision07-August-2007 RFH
          SynonymsAnti-MAPK1/2, Activated
          ApplicationELISA (0.05 µg/ml)
          Immunoblotting (1 µg/ml, colorimetric or 0.1 µg/ml, chemiluminescence)
          Immunoprecipitation (1-10 µg/10⁶ vanadate-treated A431 cells)
          DescriptionMouse monoclonal antibody purified from serum-free cell culture supernatant by thiophilic adsorption and size exclusion chromatography. Recognizes the ~42-44 kDa ERK1 and ERK2 protein phosphorylated on Thr202/Tyr204. Supplied with control lysate consisting of 1 x 106 vanadate-treated A431 cells.
          HostMouse
          Immunogen speciesHuman
          Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Thr204/204 phosphorylation stie of mouse ERK1 and ERK2
          Clone12D4
          IsotypeIgG₁
          Specieshuman, mouse, rat
          Positive controlvanadate-treated A431 cells (supplied)
          FormLyophilized
          Formulation100 µg antibody lyophilized from 2X PBS, sucrose, polyethylene glycol (PEG) and 150 µl lyophilized control lysate from 1 x 10⁶ vanadate-treated A431 cells.
          Preservative≤0.1% sodium azide (antibody only)
          CommentsAntibody requires phosphorylation at both the Thr and Tyr site for recognition. Does not recognize non-phosphorylated MAP kinase 1/2. Does not cross-react with SAPK 1 or SAPK2 proteins. Variables associated with assay conditions will dictate the proper working dilution.
          Storage -20°C
          Do Not Freeze Ok to freeze
          Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute the positive control cell lysate with 200 µl dH₂O. Following complete solubilization add 200 µl 2X SDS-PAGE sample buffer and incubate at 90°C for 5 min. Aliquot and freeze (-20°C). Avoid freeze/thaw cycles of reconstituted antibody and control lysate.
          Toxicity Standard Handling

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          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies